EC Number   |
Reference  |
|---|
   3.4.22.53 | coexpression from large-subunit and small-subunit plasmids in Escherichia coli strain BL21(DE3) |
643968 |
| 3.4.22.53 | isolation of cDNA clone for the 80000 Da subunit |
643970 |
| 3.4.22.53 | cDNA fragments corresponding to the domains with E-F hand structures in the large and small subunits are inserted into an expression vector pIC18 or pUC8. The resulting plasmids are used to transform Escherichia coli and isopropyl-1-thio-beta-D-galactoside-inducible expression is induced |
643971 |
| 3.4.22.53 | a 629 bp fragment is cloned into the EcoRV site of pBluescript II KS+ vector |
643972 |
| 3.4.22.53 | cloning of the cDNA for the large subunit |
644009 |
| 3.4.22.53 | functional analysis of the upstream region of the gene for the large subunit by means of transient expression assay on HeLa cells using chloramphenicol transferase constructs identifies four negative regulatory regions tandemly reiterated just upstream of the promoter region, P1 and P2 |
644010 |
| 3.4.22.53 | - |
643974, 644011, 685818 |
| 3.4.22.53 | molecular cloning of the cDNA for the 80000 Da subunit and expression in Escherichia coli |
644016 |
| 3.4.22.53 | the bacterial production of recombinant rat calpain II is improved greatly by the use of two compatible plasmids for the two subunits. The calpain small subunit C-terminal fragment is expressed from a new A15-based vector created by cloning T7 contol elements into pACYC177. This vector is compatible with the ColE1-based pET-24d(+) vector containing the calpain large subunit and the yield of calpain activity is increased at least 16fold by coexpression from theses two vectors. A high level of activity is also obtained from a bicistronic construct containing the subunit cDNAs under the control of one T7 promoter |
644028 |
| 3.4.22.53 | m-calpain is produced in a soluble form using a baculovirus expression system |
644029 |
