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Commentary
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by complementation of a temperature-sensitive Escherichia coli lpxD mutant, a meningococcal chromosomal fragment is cloned that carries the lpxD homologue
gene AtlpxD, two AtLpxD isozymes, AtLxpD1 and AtLpxD2, are encoded on chromosome 4, DNA and amino acid sequence determination and analysis, expression as GFP-tagged protein in Arabidopsis thaliana
gene lpxD, expression as His6-tagged protein in Escherichia coli strain BL21(DE3)
gene lpxD, expression of N-terminally His6-tagged LpxD in Escherichia coli strain BL21 (DE3)
genes lpxD1 and lpxD2, DNA and amino acid sequence determination and analysis, phyylogenetic analysis, the lpxD1gene lies within an essential four-gene operon, lpxD1/fabZ/lpxA/lpxB, while the second gene lpxD2 as a single gene is predicted to be regulated by its own promoter
lpxA (lpxAPg) and lpxDPg are cloned and expressed in Escherichia coli strains in which the homologous gene is mutated. Lipid A from strains expressing either of the Porphyromonas gingivalis transferases contains 16-carbon hydroxy fatty acids in addition to the normal Escherichia coli 14-carbon hydroxy fatty acids, demonstrating that these acyltransferases display a relaxed acyl chain length specificity
LpxD protein modified with an N-terminal His6 tag followed by a one glycine residue linker and the P2A substitution, is constructed and transformed into Escherichia coli Rosetta (DE3)/pLysS
overexpression in Escherichia coli
when the wild-type firA gene is cloned into a T7-based expression vector, N-acyltransferase specific activity increases almost 360fold relative to wild-type extracts
Results 1 - 10 of 10