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Results 1 - 6 of 6
EC Number
Commentary
Reference
expression in Escherichia coli
gene dbat, functional expression in Escherichia coli strain JM109, co-expression with acetyl-CoA synthase, gene acs, EC 6.2.1.1, or with acetoacetate:acetyl-CoA CoA-transferase, genes atoAD of the ato operon, EC 2.8.3.8, expressed from and regulated by genes encoded on the bacterial chromosome, overview; into the vector pCWori+ for expression in Escherichia coli JM109 and BL21DE3 cells
into the TA cloning vector pMD19-T for sequencing
into the TA cloning vector pMD19-T for sequencing; into the TA cloning vector pMD19-T for sequencing
overexpression in Escherichia coli. Three kinds of fusion systems with isopropyl-beta-D-1-thiogalactopyranoside inducible promoters are used. The fusion partners are glutathione S-transferase tag, hexahistidine fusion tag, and a biotinylated peptide tag
the amplified PCR fragment is subcloned into the pGEM-T Easy vector and subsequently into the pET32a+ vector for expression in Escherichia coli BL21DE3 cells
Results 1 - 6 of 6