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C-subunit expressed as GST-fusion protein in Escherichia coli BL21
gene cluster clrABDC, genetic organization, chlorate reductase is cotranscribed with cytochrome c, UniProt ID B3VLA9, and other downstream genes in the gene cluster for chlorate respiration of Ideonella dechloratans. In vivo production of a single polycistronic transcript covering eight open reading frames including clrABDC, cyc, mobB and arsR. Transcription levels of the cyc and clrA genes are compared to each other by the use of qRT-PCR in RNA preparations from cells grown under aerobic or chlorate reducing anaerobic conditions. The two genes show the same mRNA levels under both growth regimes, indicating that no transcription termination occurs between them. Higher transcription levels are observed at growth without external oxygen supply. Quantitative real-time RT-PCR expression analysis
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