EC Number |
---|
1.1.2.4 | - |
1.1.2.4 | gene amplification in Escherichia coli |
1.1.2.4 | gene DLD, expression of C-terminally GFP-tagged full-length enzyme under control of the CaMV35S promoter in Nicotiana benthamiana leaf epidermi. Overexpression of D-LDH ameliorates the growth of wild-type plants in the presence of D-lactate and methylglyoxal and reverses the increased sensitivity of dldh loss-of-function lines to these compounds |
1.1.2.4 | gene DLD1, 6fold overexpression of DLDH under the strong constitutive promoter prAOX in Hansenula polymorpha strain DELTAcyb2 tr6, a strain with impairment in glucose repression of AOX promoter and devoid of catalase and L-lactate-cytochrome c oxidoreductase activities |
1.1.2.4 | gene DLD1, recombinant expression in L-lactate-cytochrome c oxidoreductase CYB2 deficient Hansenula polymorpha strain NCYC495 leu1-1 is used for recombinant overexpression of the His6-tagged enzyme using the gene with a terminator region driven by the alcohol oxidase gene promoter. The mutant strain shows increased DlDH activity and abolished activity of flavocytochrome b2, EC 1.1.2.3 |
1.1.2.4 | in Saccharomyces cerevisiae, gene amplification in Escherichia coli |
1.1.2.4 | into vector pET16 and expressed in Escherichia coli BLR DE3 pLysS |