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Results 1 - 7 of 7
EC Number
Cloned (Commentary)
gene amplification in Escherichia coli
gene DLD, expression of C-terminally GFP-tagged full-length enzyme under control of the CaMV35S promoter in Nicotiana benthamiana leaf epidermi. Overexpression of D-LDH ameliorates the growth of wild-type plants in the presence of D-lactate and methylglyoxal and reverses the increased sensitivity of dldh loss-of-function lines to these compounds
gene DLD1, 6fold overexpression of DLDH under the strong constitutive promoter prAOX in Hansenula polymorpha strain DELTAcyb2 tr6, a strain with impairment in glucose repression of AOX promoter and devoid of catalase and L-lactate-cytochrome c oxidoreductase activities
gene DLD1, recombinant expression in L-lactate-cytochrome c oxidoreductase CYB2 deficient Hansenula polymorpha strain NCYC495 leu1-1 is used for recombinant overexpression of the His6-tagged enzyme using the gene with a terminator region driven by the alcohol oxidase gene promoter. The mutant strain shows increased DlDH activity and abolished activity of flavocytochrome b2, EC 1.1.2.3
in Saccharomyces cerevisiae, gene amplification in Escherichia coli
into vector pET16 and expressed in Escherichia coli BLR DE3 pLysS
Results 1 - 7 of 7