DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli
DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain OM17
expressed in Escherichia coli BL21(DE3)
expression in Escherichia coli
expression in Escherichia coli. Introduction of homocitrate synthase, homoaconitase and homoisocitrate dehydrogenase from Saccharomyces cerevisiae into Escherichia coli enables +1 carbon extension from 2-oxoglutarate to 2-oxoadipate, which is subsequently converted into glutarate by a promiscuous 2-oxo acid decarboxylase (KivD) and a succinate semialdehyde dehydrogenase (GabD)
expression of His-tagged enzyme in Escherichia coli strain TOP 10F'
LYS1 gene codes for the alpha-aminoadipate pathway enzymes in fungi, can complement LYS1-deficient mutants of Candida albicans and Saccharomyces cerevisiae, amplification in Escherichia coli
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)