EC Number |
Reference |
---|
1.1.1.365 | expressed in Escherichia coli BL21 cells |
715098 |
1.1.1.365 | expressed in Escherichia coli DH5alpha cells |
722316 |
1.1.1.365 | expressed in Saccharomyces cerevisiae strain CEN.PK2-1B |
724270 |
1.1.1.365 | expressed in Solanum tuberosum cultivar Taedong Valley |
725116, 726236 |
1.1.1.365 | expressed in Solanum tuberosum via Agrobacterium tumefaciens-mediated transformation |
725952 |
1.1.1.365 | expressed in Arabidopsis thaliana and in Escherichia coli XL1-Blue MRF' cells |
726022 |
1.1.1.365 | gene GalUR, real-time quantitative PCR enzyme expression analysis |
741550 |
1.1.1.365 | gene GalUR, recombinant stable overexpression in Solanum lycopersicum cv. Zhongshu 4 leaves and fruits via Agrobacterium tumefaciens strain C58C1 transfection method using the pCB302-3 vector and CaMV 35S promoter, reverse transcriptase-PCR expression analysis. The transgenic plants show 2.2-2.5fold increased enzyme activity compared to wild-type |
741553 |
1.1.1.365 | gene gaaA, expression analysis in wild-type strain and in a strain overexpressing the D-galacturonic acid-specific GatA transporter |
741576 |
1.1.1.365 | ORF FaGalUR is recombinantly expressed in Solanum lycopersicum from plasmid pPGGUSI, which contains a 4.8 Kb promoter fragment of the polygalacturonase (PG) gene and its terminator, or from a plasmid containing the 35S CaMV promoter, mobilized from Escherichia coli by triparental mating, via Agrobacterium tumefaciens strain LBA4404 in tomato plants, PCR expression analysis. FaGalUR expression driven by the CaMV 35S promoter causes the expression of the gene in most parts of the plants, while in the second, FaGalU, is expressed under the control of the tomato fruit ripening-specific PG promoter. All of the transgenic lines are morphologically indistinguishable over different generations from control lines both in vegetative traits, such as leaf size or plant height, and fruit traits such as color or size. The majority of transgenic plants display a slight increase in fruit yield, up to 1.4fold, which is a consequence of an increase in the number of fruits rather than an increase in fruit weight. No significant changes in soluble solids of transgenic plants, but a reduction in acidity |
742163 |