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EC Number
Commentary
Reference
expressed in Arabidopsis thaliana and in Escherichia coli XL1-Blue MRF' cells
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli DH5alpha cells
expressed in Saccharomyces cerevisiae strain CEN.PK2-1B
expressed in Solanum tuberosum cultivar Taedong Valley
expressed in Solanum tuberosum via Agrobacterium tumefaciens-mediated transformation
gene gaaA, expression analysis in wild-type strain and in a strain overexpressing the D-galacturonic acid-specific GatA transporter
gene GalUR, real-time quantitative PCR enzyme expression analysis
gene GalUR, recombinant overexpression in Solanum lycopersicum cv. Ailsa Craig leaves and fruits via Agrobacterium tumefaciens strain C58C1 transfection resulting in 2fold and 1.6fold increase in ascorbate level in tomato fruit and leaf, respectively, which correlates positively with FaGalUR transcriptional abundance and enzyme GalUR activity compared to wild-type plants, real-time PCR expression analysis
gene GalUR, recombinant stable overexpression in Solanum lycopersicum cv. H15 leaves and fruits using Agrobacterium tumefaciens strain EHA 105 transfection method, the pNW2300 vector, and the CaMV35S promoter. The transgenic plants show increased enzyme content and activity compared to wild-type. Semi-quantitative RT-PCR expression analysis, increased ascorbic acid content is correlated with high level of transcript expression, that is 2-3fold increased compared to wild-type
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