EC Number   |
Reference |
|---|
   6.3.2.30 | gene cphA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional overexpression of His-tagged Tlr2170 in Escherichia coli BL21(DE3) |
690592 |
| 6.3.2.30 | gene cphA, expression in Solanum tuberosum tubers under control of the tuber-specific class 1 promoter B33 directed to tuber cytosol or to tuber plastids leading to cyanophycin accumulation in the tubers |
706150 |
| 6.3.2.30 | gene cphA, subcloning and expression of His-tagged wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3) |
691115 |
| 6.3.2.30 | gene cphA49 cloned from deep-sea sediment metagenomic library with degenerated primers, amplified from the fss49 fosmid by PCR, DNA and amino acid sequence determination and analysis, functional expression of His-tagged cphA49 in Escherichia coli strain BL21(DE3), phylogenetic tree, subcloning in Escherichia coli strain DH5alpha |
726784 |
| 6.3.2.30 | gene cphA6308, expression in Pichia pastoris and Escherichia coli from expression vectors pPICHOLI-C with the copper-inducible CUP1 promoter and pPICHOLI-3 with the methanol-inducible AOX1 promoter. Stabilization of the expression plasmid, the his4 gene from Saccharomyces cerevisiae is introduced and the expression is made in the His auxotrophic Pichia pastoris strain GS115. Recombinant polymer production by wild-type and mutant enzymes and product compositions, overview |
701820 |
| 6.3.2.30 | gene cphA6308, functional expression in Saccharomyces cerevisiae strains G175 and BY4741, which is much more efficient with the copper ion-inducible CUP1 promoter instead of the GAL1 promoter, the yeast strains produce water-soluble and water-insoluble cyanophycin polymer. Growth of transgenic yeasts in the presence of 15 mM lysine results in an incorporation of up to 10 mol% of lysine into cyanophycin, overview. Subcloning in Escherichia coli strain XL1-Blue |
690533 |
| 6.3.2.30 | gene cphA6803, enzyme expression in auxotrophic mutant Rhizopus oryzae strain M16 under control of the pyruvate decarboxylase promoter and terminator elements of Rhizopus oryzae by biolistic transformation method |
726759 |
| 6.3.2.30 | gene cphA7120, enzyme expression in auxotrophic mutant Rhizopus oryzae strain M16 under control of the pyruvate decarboxylase promoter and terminator elements of Rhizopus oryzae by biolistic transformation method |
726759 |
