EC Number |
Reference |
---|
4.1.1.18 | gene cadA, recombinant induced expression of lysine decarboxylase in Escherichia coli strain BL21(DE3) for the high-level production of cadaverine from industrial grade L-lysine |
746801 |
4.1.1.18 | gene cadA, sequence comparisons and phylogenetic analysis, recombinant functional expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), the activity of purified CadA from Klebsiella pneumoniae is lower than that of CadA from Escherichia coli, the activity of Klebsiella pneumoniae CadA in whole-cell bioconversion is comparable to that of Escherichia coli CadA, resulting in 90% lysine conversion of L-lysine to cadaverine with cofactor pyridoxal 5'-phosphate |
748382 |
4.1.1.18 | gene gtLDC, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha |
748513 |
4.1.1.18 | gene ldc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of the soluble His-tagged enzyme in Escherichia coli strain BL21(DE3), quantitative real-time reverse transcription PCR enzyme expression analysis |
749226 |
4.1.1.18 | gene ldc, recombinant expression of codon-optimized enzyme in Escherichia coli K-12 resulting in strain LN20, optimizing the sequence located in the region of the ribosome-binding site in order to enhance translation efficiency further improves the system, subcloning in Escherichia coli TOP10 cells. Batch and fed-batch production of Klebsiella oxytoca lysine decarboxylase from several modifed strains, overview |
747655 |
4.1.1.18 | gene ldc, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JM109/pTrc99a-ldc2-41 |
747358 |
4.1.1.18 | gene ldc, recombinant expression of wild-type enzyme and codon-optimized enzyme in Escherichia coli K-12 resulting in strains LN18 and LN20, respectively. Batch and fed-batch production of Klebsiella oxytoca lysine decarboxylase from several modifed strains, overview |
747357 |
4.1.1.18 | gene ldcC, recombinant constitutive expression of lysine decarboxylase in Escherichia coli strain BL21(DE3) for the high-level production of cadaverine from industrial grade L-lysine |
746801 |
4.1.1.18 | gene ldcC, recombinant expression in Escherichia coli strain BL21(DE3), optimization of conditions for enzyme overexpression, e.g. with respect to pH, cadaverine concentration, and IPTG concentration, overview |
748380 |
4.1.1.18 | gene ldcC, recombinant overexpression in Escherichia coli strain BL21(DE3) in inclusion bodies with 3-10 mg/ml protein in batch cultivation. The aggregated enzyme EcLDCc-CatIBs is catalytically active |
749366 |