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Cloned (Commentary)
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3.5.99.7
subcloned into pET-11d and expressed in Escherichia coli
648260
3.5.99.7
Using non-transformed canola (Brassica napus) or canola transformed with the ACC deaminase gene from Pseudomonas putida UW4. The transformed canola line has two copies of the ACC deaminase gene under the control of the rootspecific Agrobacterium rhizogenes promoter. This homozygous line is created through Agrobacterium tumefaciens transformation of canola callus culture.
691950
3.5.99.7
wild-type enzyme and mutants Y268F and Y294F as His6-tagged proteins in Escherichia coli
718894
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