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EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.53m-calpain is produced in a soluble form using a baculovirus expression system 644029
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.53molecular cloning of the cDNA for the 80000 Da subunit and expression in Escherichia coli 644016
Display the word mapDisplay the reaction diagram Show all sequences 3.4.22.53the bacterial production of recombinant rat calpain II is improved greatly by the use of two compatible plasmids for the two subunits. The calpain small subunit C-terminal fragment is expressed from a new A15-based vector created by cloning T7 contol elements into pACYC177. This vector is compatible with the ColE1-based pET-24d(+) vector containing the calpain large subunit and the yield of calpain activity is increased at least 16fold by coexpression from theses two vectors. A high level of activity is also obtained from a bicistronic construct containing the subunit cDNAs under the control of one T7 promoter 644028
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