3.4.22.10 | production of large quantities of soluble streptopain using the autoinduction method for Escherichia coli recombinant protein expression with a C-terminal His6 tag. Soluble streptopain is obtained as mature enzyme, indicating that at some point during autoinduction or cell lysis, the protease must have become active. If incubated long enough after lysis, the protease digestes most proteins in the lysate |
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