EC Number   |
Reference |
|---|
   1.14.14.3 | ligated into pET21-b vector, expressed from pZCH2 in Escherichia coli BL21 (lambdaDE3) cell line |
704579 |
| 1.14.14.3 | luxCDABE operon, genetic organization, overview |
711341 |
| 1.14.14.3 | overexpression in Escherichia coli |
348606 |
| 1.14.14.3 | overexpression of mutant in XL1 blue MRF' cell line |
348608 |
| 1.14.14.3 | overexpression of wild-type and mutant enzymes in Escherichia coli strain JM101 |
671978 |
| 1.14.14.3 | stable expression, using a bicistronic expression vector, of wild type luxA and luxB, WTA/WTB, codon-optimized luxA and wild type luxB, COA/WTB, and codon-optimized versions of both luxA and luxB genes, COA/COB, in HEK-293 cells, expression analysis, method evaluation and optimization, highest bioluminescence by expression of both codon-optimized genes, overview |
675217 |
| 1.14.14.3 | the bacterial luciferase lux gene cassette consists of five genes, luxCDABE. The lux operon is re-synthesized through a process of multibicistronic, codon-optimization to demonstrate self-directed bioluminescence emission in a mammalian HEK-293 cell line in vitro and in vivo, overview. To overcome the limitations by FMNH2 supply, co-expression of a constitutively expressed flavin reductase gene frp from Vibrio harveyi is performed leading to a 151fold increased increase in bioluminescence in cells expressing mammalian codon-optimized luxCDE and frp genes |
713371 |
