EC Number   |
Reference  |
|---|
    3.1.2.22 | expression of the enzyme in Caco-2 cells, orientation to the basolateral side, also after endocytosis of the recombinant enzyme into Caco-2 cells |
651451 |
| 3.1.2.22 | expression of wild-type and enzymes with naturally occurring mutations in COS cells as His-tagged enzyme, and in Sf9 cells, the latter via baculovirus infection |
651450 |
| 3.1.2.22 | expression of wild-type and mutant in COS-1 cells |
653651 |
| 3.1.2.22 | fragment corresponding to the entire coding region of PPT1 amplified from pCMV5-hPPT1, and cloned into XhoI sites in the polylinker region of expression vector pMSXND1, overexpressed in CHO cells. PPT1 injected intravenously into PPT1-deficient mice |
709975 |
| 3.1.2.22 | gene ppt, DNA sequence determnation and analysis, identification of 8 natural mutations of different origins leading to enzyme deficiency |
651456 |
| 3.1.2.22 | GST-fusion protein expressed in Escherichia coli BL21, expressed in HEK-293 cell |
707548 |
| 3.1.2.22 | overexpressed in Drosophila melanogaster |
710070 |
| 3.1.2.22 | overexpression of PPT2 in an insect-baculovirus expression system, secretion of the recombinant enzyme to the culture medium, expression of mutant PPT2 in COS cells, subcloning in Escherichia coli XL1-blue |
652459 |
| 3.1.2.22 | PPT1-deficient fibroblasts stably transfected with cDNA encoding PPT1 |
707557 |
| 3.1.2.22 | PPT1-KO mice are generated by gene targeting in ES cells |
693353 |
