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EC Number
Commentary
Reference
expression of the enzyme in Caco-2 cells, orientation to the basolateral side, also after endocytosis of the recombinant enzyme into Caco-2 cells
expression of wild-type and enzymes with naturally occurring mutations in COS cells as His-tagged enzyme, and in Sf9 cells, the latter via baculovirus infection
expression of wild-type and mutant in COS-1 cells
fragment corresponding to the entire coding region of PPT1 amplified from pCMV5-hPPT1, and cloned into XhoI sites in the polylinker region of expression vector pMSXND1, overexpressed in CHO cells. PPT1 injected intravenously into PPT1-deficient mice
gene ppt, DNA sequence determnation and analysis, identification of 8 natural mutations of different origins leading to enzyme deficiency
GST-fusion protein expressed in Escherichia coli BL21, expressed in HEK-293 cell
overexpressed in Drosophila melanogaster
overexpression of PPT2 in an insect-baculovirus expression system, secretion of the recombinant enzyme to the culture medium, expression of mutant PPT2 in COS cells, subcloning in Escherichia coli XL1-blue
PPT1-deficient fibroblasts stably transfected with cDNA encoding PPT1
PPT1-KO mice are generated by gene targeting in ES cells
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