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<< < Results 11 - 17 of 17
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gene LAR1, cloned from leaves, DNA and amino acid sequence determination and analysis, genotyping, the three LAR loci, FeLAR1, FeLAR2, and FeLAR3, are not genetically linked, phylogenetic analysis; gene LAR2, cloned from leaves, DNA and amino acid sequence determination and analysis, genotyping, the three LAR loci, FeLAR1, FeLAR2, and FeLAR3, are not genetically linked, phylogenetic analysis; gene LAR3, cloned from leaves, DNA and amino acid sequence determination and analysis, genotyping, the three LAR loci, FeLAR1, FeLAR2, and FeLAR3, are not genetically linked, phylogenetic analysis
gene LAR1, quantitative real-time PCR enzyme expression analysis, recombinant expression in Nicotiana tabacum cv. Petite Havana SR1 using the transfection method with Agrobacterium tumefaciens strain GV3101 and under control of the CaMV 35S promoter. The proanthocyanidin contents in either white- or pale pink-colored transgenic flowers are significantly lower than that of wild-type flowers. In contrast, both pale-pink and white flowers of the transgenic lines accumulate slightly higher levels of epicatechin than wild-type flowers, but the changes do not reach statistical significance. No significant change in catechin content is observed between wild-type flowers and either white- or pale pink-colored transgenic flowers, phenotypes, overview; gene LAR, quantitative real-time PCR enzyme expression analysis
gene LAR3, DNA and amino acid sequence determination, genotyping, genetic organization and allelic structure of the gene, identification of nonsynonymous substitutions, and to analysis of motifs in the promoter, recombinant expression in Nicotiana benthamiana, and quantitative RT-PCR analyis of allele-specific gene expression of the PaLAR3 alleles
gene LARa, complementary DNA library construction, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, functional expression of His6-tagged enzyme in Escherichia coli strain M15. Recombinant ectopic expression of CsLAR leads to the accumulation of low levels of proanthocyanidin precursors and their conjugates in Medicago truncatula hairy roots and anthocyanin-overproducing Nicotiana tabacum, but levels of oligomeric proanthocyanidins are very low. The expression of CsLAR in tobacco overproducing anthocyanin leads to the accumulation of higher levels of epicatechin and its glucoside than of catechin, phenotype and flavonoid compounds contents, overview
gene LARa, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strains EHA105 and C58C1?mediated transformation, quantitative real?time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco; gene LARb, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strains EHA105 and C58C1-mediated transformation, quantitative real-time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco; gene LARc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strain s EHA105 and C58C1-mediated transformation, quantitative real-time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco; gene LARc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strain s EHA105 and C58C1?mediated transformation, quantitative real?time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco
genes Vv lar1 and Vv lar2, quantitative real-time RT-PCR enzyme expression analysis; genes Vvlar1 and Vvlar2, quantitative real-time RT-PCR enzyme expression analysis
recombinantly expressed in Escherichia coli
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