EC Number |
Subunits |
Reference |
---|
4.1.1.88 | heterotetramer |
x-ray crystallography |
748747 |
4.1.1.88 | More |
enzyme complex is formed by subunits alpha, beta, gamma, delta. The mdcA gene product, the alpha subunit, is malonate/acetyl-CoA transferase and the mdcD gene product, the beta subunit, is malonyl-CoA decarboxylase. The mdcE gene product, the gamma subunit, may play a role in subunit interaction to form a stable complex or as a codecarboxylase. The mdcC gene product, the delta subunit, is an acyl-carrier protein |
-, 686494 |
4.1.1.88 | More |
enzyme is encoded by a gene cluster consisting of the eight consecutive genes mdcABCDEFCH and the divergently oriented mdcR gene. The mdcA, C, D and E genes encode subunits alpha, beta, gamma and delta of malonate decarboxylase. MdcA represents the ACP-transferase and that MdcD and E together probably function as malonyl-S-acyl carrier protein decarboxylase. MdcC is the (apo) acyl carrier protein subunit. MdcB and MdcG are involved in the synthesis and attachment of the prosthetic group. MdcH is probably involved in the initial activation of the enzyme by malonylation. MdcF is a membrane protein that could function as a malonate carrier |
686492 |
4.1.1.88 | More |
mdcA gene encodes the alpha subunit carrying acyl carrier protein transferase activity. The mdcD gene encodes the delta subunit, the acyl carrier protein. mdcB encodes the beta subunit, the catalytic subunit of decarboxylation of malonate. The mdcC gene encodes the gamma subunit involved in decarboxylation of the malonyl-S-acyl carrier protein |
685309 |
4.1.1.88 | More |
presence of two forms of the enzyme: a catalytically inactive SH-enzyme and the catalytically active acetyl-S-enzyme which is formed by post-translational acetylation of the SH-enzyme with ATP, acetate and a specific ligase. the delta subunit is the acyl carrier protein of the enzyme complex |
686490 |
4.1.1.88 | More |
subunit acyl carrier protein contains a 2'-(5''-phosphoribosyl)-3'-dephospho-CoA prosthetic group attached via a phosphodiester to residue S25. Formation of this phosphodiester bond is catalyzed by a phosphoribosyl-dephospho-coenzyme A transferase MdcG. Site-directed mutagenesis of D134 and/or D136 of MdcG to alanine abolishes the transfer of the prosthetic group to apo acyl carrier protein |
685057 |
4.1.1.88 | multimer |
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit |
687489 |
4.1.1.88 | multimer |
2 * 65000, alpha subunit, 1 * 32000, beta subunit, 1 * 25000, gamma subunit, 1 * 11000, delta subunit |
687464 |
4.1.1.88 | multimer |
2 * 65000, alpha subunit, 1 * 33000, beta subunit, 1 * 30000, gamma subunit, 1 * 11000, delta subunit |
687464 |
4.1.1.88 | tetramer |
1 * 65000, alpha subunit, 1* 34000, beta-subunit, 1 * 30000, gamma subunit, 1 * 12000, delta subunit, SDS-PAGE |
686490 |