EC Number   |
Subunits |
Reference |
|---|
   3.1.11.5 | dimer |
1 * 135000 + 1 * 141000, addA and addB gene encode two subunits of enzyme, the enzyme carries two homologous nuclease domains |
653270 |
| 3.1.11.5 | dimer |
1 * 140000 + 1 * 128000, dissociation by boiling, SDS-PAGE |
133995, 133996 |
| 3.1.11.5 | dimer |
1 * 155000 + 1 * 140000, protease inhibitor phenylmethylsulfonyl flouride was added |
133995 |
| 3.1.11.5 | dimer |
1 * 170000 + 1 * 60000, glycerol gradient sedimentation |
133995 |
| 3.1.11.5 | heterotrimer |
complex of three RecB, RecC, RecD proteins |
653270 |
| 3.1.11.5 | heterotrimer |
RecB, RecC and RecD subunits, The nuclease active site on the RecB 30000 Da domain is the universal nuclease active site of enzyme that is responsible for single-stranded DNA degradation in both directions. |
652076 |
| 3.1.11.5 | heterotrimer |
RecB, RecC and RecD, The C-terminus of RecC: 35000 Da, is required for assembly of the RecD subunit into enzyme holoenzyme but not for recombination proficiency. The N-terminus of RecC: 95000 Da, contains functions essential for recombination and is also required for chi activity. RecD is essential for nuclease activity, regulation by the recombination hotspot Chi, and high affinity for DNA ends. RecC interacts with RecD to form a heterotrimer only in the presence of RecB. |
651426 |
| 3.1.11.5 | More |
structure of the SH3-fold RecD 2B domain, overview |
750403 |
| 3.1.11.5 | More |
the difference between the AddAB and RecBCD enzymes is that the two enzyme have a very different subunit composition, the AddA subunit is a direct homologue of the RecB subunit, the helicases of each complex |
653270 |
| 3.1.11.5 | pentamer |
1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor |
133995, 134021 |
