EC Number   |
Subunits |
Reference |
|---|
    2.5.1.56 | ? |
x * 43300, His-tagged recombinant enzyme, MALDI-TOF MS |
657851 |
| 2.5.1.56 | dimer |
2 * 38987, electrospray ionisation mass spectrometry |
637325 |
| 2.5.1.56 | dimer |
2 * 39000, SDS-PAGE |
671716 |
| 2.5.1.56 | dimer |
2 * 52000, SDS-PAGE |
-, 637321 |
| 2.5.1.56 | homodimer |
2 * 38935, sequence calculation, 2 * 38000, about, recombinant enzyme, SDS-PAGE |
737983 |
| 2.5.1.56 | homodimer |
2 * 45000, about, recombinant enzyme, SDS-PAGE, 2 * 41000, about, recombinant enzyme, HPLC/MS/ESI |
-, 738339 |
| 2.5.1.56 | homodimer |
the enzyme comprises two distinct domains, an N-terminal catalytic (beta/alpha)8 barrel linked to a C-terminal antifreeze protein-like (AFPL) domain. Loss of the AFPL domain destabilizes the dimeric form of the enzyme nd renders it inactive. The AFPL domain plays a critical role for both the catalytic function and quaternary structure stability of N-acetylneuraminic acid synthase. The enzyme mutant G272Term lacking the AFPL domain is partly monomeric |
-, 739614 |
| 2.5.1.56 | monomer |
1 * 38629, calculated from amino acid sequence |
671716 |
| 2.5.1.56 | monomer |
1 * 38649, ESI-MS |
671716 |
| 2.5.1.56 | More |
structure comparisons |
737983 |
