EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
  6.3.4.22 | ATP + agmatine + [tRNAIle2]-cytidine34 |
- |
Archaeoglobus fulgidus |
[tRNAIle2]-2-agmatinylcytidine34 + ? |
- |
? |
| 6.3.4.22 | ATP + agmatine + [tRNAIle2]-cytidine34 |
- |
Pyrococcus horikoshii |
[tRNAIle2]-2-agmatinylcytidine34 + ? |
- |
? |
| 6.3.4.22 | ATP + agmatine + [tRNAIle2]-cytidine34 |
the enzyme catalyzes the essential modification at the anticodon wobble position (position 34) of the AUA-codon of tRNAIle |
Archaeoglobus fulgidus |
[tRNAIle2]-2-agmatinylcytidine34 + AMP + diphosphate |
- |
? |
| 6.3.4.22 | ATP + agmatine + [tRNAIle2]-cytidine34 |
the precursor form of tRNAIle bearing an unmodified cytidine at the wobble position (C34) is aminoacylated with methionine (Met) and decodes the AUG codon.The Thr18-Cyt34 kinase domain first hydrolyzes ATP into AMP and diphosphate, then phosphorylates the C2 position of cytidine34 with the gamma-phosphate. Next, the amino group of agmatine attacks this position to release the phosphate and form 2-agmatinylcytidine. The Thr18-Cyt34 kinase domain also autophosphorylates the Thr18 of the enzyme, which may be involved in 2-agmatinylcytidine formation. Catalytic efficiency (kcat/Km) with GTP, UTP or CTP as substrate is less than 1% compared to ATP |
Archaeoglobus fulgidus |
[tRNAIle2]-2-agmatinylcytidine34 + AMP + diphosphate |
- |
? |
