EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
  6.2.1.B4 | ATP + glycine + [acyl-carrier protein Blr6284] |
- |
Bradyrhizobium japonicum |
AMP + diphosphate + glycyl-[acyl-carrier protein Blr6284] + AMP + diphosphate |
- |
? |
| 6.2.1.B4 | ATP + glycine + [acyl-carrier protein Blr6284] |
the carrier protein Blr6284 is cloned and overexpressed in Escherichia coli (UniProt: Q89GR1). The 4'-phosphopantetheinyl prosthetic group is attached to purified apo-carrier proteins. The enzyme is homologous to the catalytic domain of highly diverged, atypical seryl-tRNA synthetases found only in methanogenic archaea and are deprived of the tRNA-binding domain. In comparison to seryl-tRNA synthetases, the homolog displays different and relaxed amino acid specificity. The homolog lacks canonical tRNA aminoacylating activity and instead transfers activated amino acid to phosphopantetheine prosthetic group of putative carrier proteins, whose genes are identified in the genomic surroundings of the homolog. Strong preference for Gly in the ATP-diphosphate exchange assay. L-Alanine is activated approximately 300fold less efficiently. No activity in ATP-diphosphate exchange assay: L-Ser, D-Ser, D-Ala, D-Pro, D-Glu |
Bradyrhizobium japonicum |
AMP + diphosphate + glycyl-[acyl-carrier protein Blr6284] + AMP + diphosphate |
- |
? |
| 6.2.1.B4 | ATP + glycine + [acyl-carrier protein Bsr0959] |
[acyl-carrier protein] Bsr0959 |
Bradyrhizobium japonicum |
AMP + diphosphate + glycyl-[acyl-carrier protein] + AMP + diphosphate |
- |
? |
| 6.2.1.B4 | ATP + glycine + [acyl-carrier protein Bsr0959] |
the carrier protein Bsr0959 is cloned and overexpressed in Escherichia coli (UniProt: Q89VT6). The 4'-phosphopantetheinyl prosthetic group is attached to purified apo-carrier proteins. The enzyme is homologous to the catalytic domain of highly diverged, atypical seryl-tRNA synthetases found only in methanogenic archaea and are deprived of the tRNA-binding domain. In comparison to seryl-tRNA synthetases, the homolog displays different and relaxed amino acid specificity. The homolog lacks canonical tRNA aminoacylating activity and instead transfers activated amino acid to phosphopantetheine prosthetic group of putative carrier proteins, whose genes are identified in the genomic surroundings of the homolog. Strong preference for Gly in the ATP-diphosphate exchange assay. L-Alanine is activated approximately 300fold less efficiently. No activity in ATP-diphosphate exchange assay: L-Ser, D-Ser, D-Ala, D-Pro, D-Glu |
Bradyrhizobium japonicum |
AMP + diphosphate + glycyl-[acyl-carrier protein] + AMP + diphosphate |
- |
? |
