EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   4.6.1.20 | 5'-(U)6-GA-(U)5-3' RNA + H2O |
digestion of the platinated 5'-(U)6-GA-(U)5-3' RNA shows a reaction product corresponding to [UUUUUUGA + Pt(NH3)2 + H],+ depicting RNase cleavage 3' to an adenosine predicted to be involved in a cisplatin diadduct. The platinated G*A* RNA adduct is uniquely recognized and processed by RNase U2, whereas other purine-Pt(II) adducts are not. The basis for this selectivity may arise from the RNase's known A - G preference, allowing the endonuclease in some cases to partially recognize the 3' platinated A despite platinum modification |
synthetic construct |
? |
- |
? |
| 4.6.1.20 | more |
YoeB toxin has in vitro Rnase activity that preferentially cleaves at the 3-end of purine ribonucleotides |
Escherichia coli |
? |
- |
? |
| 4.6.1.20 | more |
specifically recognizes purine ribonucleotides and catalyzes cleavage of the 3' phosphodiester bond attached to a G or an A. For platinated sequences containing XY = GG, AG, and GU, RNase U2 cleavage is not observed, being blocked at the purine nucleotides predicted to be involved in platinum binding |
synthetic construct |
? |
- |
? |
| 4.6.1.20 | RNA + H2O |
- |
Ustilago sphaerogena |
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates |
- |
? |
| 4.6.1.20 | RNA + H2O |
r, preferential cleavage at Ap or Gp |
Ustilago sphaerogena |
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates |
- |
? |
| 4.6.1.20 | RNA + H2O |
- |
Ustilago sphaerogena CBS 534.71 |
3'-Phosphomononucleotides + 3'-phosphooligonucleotides + 2',3'-cyclic nucleotide phosphates |
- |
? |
| 4.6.1.20 | RNA + H2O |
- |
Ustilago sphaerogena |
? |
- |
? |
| 4.6.1.20 | RNA containing adenosine 3'-(1-naphthyl phosphate) + H2O |
- |
Ustilago sphaerogena |
? |
- |
? |
| 4.6.1.20 | RNA containing adenosine 3'-(benzyl phosphate) + H2O |
- |
Ustilago sphaerogena |
? |
- |
? |
| 4.6.1.20 | RNA containing adenosine 3'-(methyl phosphate) + H2O |
- |
Ustilago sphaerogena |
? |
- |
? |
