EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   4.2.99.18 | (dA)230*(dT,dU)230 |
- |
Daucus carota |
? |
- |
? |
| 4.2.99.18 | (pT)7(p(2,3-dihydroxy-5-oxopentyl phosphate))(pT)6 |
- |
Homo sapiens |
? |
- |
? |
| 4.2.99.18 | 12-mer oligodeoxyribonucleotide containing a 2'-deoxyguanosine at the natural AP site |
- |
Homo sapiens |
? |
- |
? |
| 4.2.99.18 | 12-mer oligodeoxyribonucleotide containing a natural AP site |
the minimal kinetic model for the natural AP site incision consists of four stages corresponding to three different transient states of APE1. When the enzyme is complexed with the AP-substrate, the catalytic cycle is completed within 3 s |
Homo sapiens |
? |
- |
? |
| 4.2.99.18 | 12-mer oligodeoxyribonucleotide containing a tetrahydrofuran analogue at the natural AP site |
- |
Homo sapiens |
? |
- |
? |
| 4.2.99.18 | 18-mer containing P33-labeled tetrahydrofuran |
- |
Cricetulus griseus |
? |
- |
? |
| 4.2.99.18 | 21 bp double-stranded DNA containing an apurinic/apyrimidinic-site analogue |
- |
Geobacillus kaustophilus |
? |
the affinity of EndoIV for the substrate analogue is very high and its dissociation constant is less than 0.01 microM. A C-terminal DNA-recognition loop at residues 265-269 that is only present in the long type enzymes contributes to its high affinity for apurinic/apyrimidinic sites |
? |
| 4.2.99.18 | 26-bp-oligonucleotide |
oligonucleotide containing a 5'-hexachloro-fluorescein phosphoramidite-labeled tetrahydrofuranyl residue in the middle |
Toxoplasma gondii |
5'-hexachloro-fluorescein phosphoramidite-labeled 13-mer fragment + ? |
- |
? |
| 4.2.99.18 | 3'-fluorescein-labeled '-AGTAGACAAG(dU)TACCATGCCTGCACGAAGTT-3' |
- |
Homo sapiens |
? |
- |
? |
| 4.2.99.18 | 3'-fluorescein-labeled 5'-AACTTCGTGCAGGCATGGTAG(dU)TTGTCTACT-3' |
- |
Homo sapiens |
? |
- |
? |
