EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.7.1.14 | (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O |
- |
Escherichia coli |
(2E)-2-hydroxypenta-2,4-dienoate + succinate |
- |
? |
3.7.1.14 | (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O |
the enzyme is involved catabolism of 3-(3-hydroxyphenyl)propionate |
Escherichia coli |
(2E)-2-hydroxypenta-2,4-dienoate + succinate |
- |
? |
3.7.1.14 | (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O |
the enzyme is involved in 3-phenylpropanoate catabolism |
Escherichia coli |
(2E)-2-hydroxypenta-2,4-dienoate + succinate |
- |
? |
3.7.1.14 | (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O |
- |
Escherichia coli KK334 |
(2E)-2-hydroxypenta-2,4-dienoate + succinate |
- |
? |
3.7.1.14 | (2E,4Z)-2-hydroxy-6-oxonona-2,4-dienedioate + H2O |
the enzyme is involved in 3-phenylpropanoate catabolism |
Escherichia coli KK334 |
(2E)-2-hydroxypenta-2,4-dienoate + succinate |
- |
? |
3.7.1.14 | (2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate + H2O |
the enzyme also catalyses the reverse reaction of C-C hydrolysis, namely C-C bond formation |
Escherichia coli |
(2Z)-2-hydroxypenta-2,4-dienoate + succinate |
- |
r |
3.7.1.14 | 2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid + H2O |
MhpC mutant is able to accept the 2-hydroxy-6-keto-6-phenylhexa-2,4-dienoic acid, on a shorter time scale |
Escherichia coli |
? |
- |
? |
3.7.1.14 | more |
MCP hydrolases catalyse the C-C bond cleavage of compounds with a common structure, 2-hydroxy-6-oxohexa-2,4-dienoate with different substituents at the C-6 carbon |
Escherichia coli |
? |
- |
? |
3.7.1.14 | more |
the enzyme is able to catalyse carbon-carbon bond formation. In addition to its natural substrate 2-hydroxy-6-oxonona-1,9-dienedioic acid, enzyme MhpC also hydrolyses various analogues and also the hydrolysis of ester bonds of monoethyl adipate and 4-nitrophenyl valerate. The H114A mutant of the enzyme also hydrolyses 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid, HOPDA, a substrate of enzyme BphD, EC 3.7.1.8. Incubation of monomethyl succinate and ethyl 2-hydroxypentadienoate with the wild-type freeze-dried MhpC in hexane result in C-C bond formation product |
Escherichia coli |
? |
- |
? |