EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
  3.5.99.8 | 5-nitroanthranilate + H2O |
- |
Bradyrhizobium sp. |
5-nitrosalicylate + NH3 |
- |
? |
| 3.5.99.8 | 5-nitroanthranilate + H2O |
- |
Bradyrhizobium sp. JS329 |
5-nitrosalicylate + NH3 |
- |
? |
| 3.5.99.8 | 5-nitroanthranilate + H2O |
initial step in biodegradation of 5-nitroanthranilic acid as sole carbon, nitrogen and energy source |
Bradyrhizobium sp. |
5-nitrosalicylate + NH3 |
- |
? |
| 3.5.99.8 | more |
mechanism of release of the nitro group, overview. The nitro group is spontaneously eliminated as nitrite concomitant with the formation of a lactone from the ring fission product of 5NSA dioxygenation |
Bradyrhizobium sp. |
? |
- |
? |
| 3.5.99.8 | more |
enzyme 5NAA-A is specific for 5-nitroanthranilate (5NAA) and cannot hydrolyze other tested derivatives, i.e. 4-nitroaniline, 4-amino-3-nitrobenzoic acid, and 2-bromo-5-nitrobenzoic acid, which are likewise poor inhibitors. The enzyme employs a nucleophilic aromatic substitution mechanism |
Bradyrhizobium sp. JS329 |
? |
- |
- |
