EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.5.2.B2 | (+)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O |
- |
Saccharolobus solfataricus |
+ (+)-4-amino-cyclopent-2-enecarboxylic acid |
- |
? |
3.5.2.B2 | (+)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O |
- |
Saccharolobus solfataricus ATCC 35092 |
+ (+)-4-amino-cyclopent-2-enecarboxylic acid |
- |
? |
3.5.2.B2 | (1S,4R)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O |
- |
Microbacterium hydrocarbonoxydans |
(1R,4S)-4-aminocyclopent-2-ene-1-carbaldehyde |
- |
? |
3.5.2.B2 | (1S,4R)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O |
- |
Saccharolobus solfataricus |
(1R,4S)-4-aminocyclopent-2-ene-1-carbaldehyde |
- |
? |
3.5.2.B2 | (1S,4R)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O |
- |
Delftia acidovorans |
(1R,4S)-4-aminocyclopent-2-ene-1-carbaldehyde |
- |
? |
3.5.2.B2 | (1S,4R)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O |
- |
Saccharolobus solfataricus |
(1R,4S)-4-aminocyclopent-2-ene-1-carbaldehyde |
50% yield. When racemic 2-azabicyclo[2.2.1]hept-5-en-3-one is used, (1R,4S)-2-azabicyclo[2.2.1]hept-5-en-3-one is produced with 99% enantiomeric excess |
? |
3.5.2.B2 | (1S,4R)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O |
- |
Aeropyrum pernix |
(1R,4S)-4-aminocyclopent-2-ene-1-carbaldehyde |
enzyme specifically hydrolyzes the (+)-lactam, (1S,4R)-2-azabicyclo[2.2.1]hept-5-en-3-one, without any actin against the (-)-isomer. Yield is 48%, 97% enantiomeric excess |
? |
3.5.2.B2 | (1S,4R)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O |
enantiospecific cleavage of (1S,4R)-isomer |
Bradyrhizobium japonicum |
(1R,4S)-4-aminocyclopent-2-ene-1-carbaldehyde |
99.8% enantiomeric excess |
? |
3.5.2.B2 | (1S,4R)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O |
- |
Delftia acidovorans DSM 14801 |
(1R,4S)-4-aminocyclopent-2-ene-1-carbaldehyde |
- |
? |
3.5.2.B2 | (1S,4R)-2-azabicyclo[2.2.1]hept-5-en-3-one + H2O |
enantiospecific cleavage of (1S,4R)-isomer |
Bradyrhizobium japonicum USDA 6 |
(1R,4S)-4-aminocyclopent-2-ene-1-carbaldehyde |
99.8% enantiomeric excess |
? |