EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
3.5.1.78 | gamma-Glu-Ala-Gly-4-nitroanilide + H2O |
- |
Escherichia coli |
? |
- |
? |
3.5.1.78 | glutathionylspermidine + H2O |
- |
Escherichia coli |
glutathione + spermidine |
- |
? |
3.5.1.78 | glutathionylspermidine + H2O |
- |
Crithidia fasciculata |
glutathione + spermidine |
- |
r |
3.5.1.78 | glutathionylspermidine + H2O |
- |
Escherichia coli K-12 |
glutathione + spermidine |
- |
? |
3.5.1.78 | glutathionylspermidine + H2O |
- |
Escherichia coli |
glutathione + spermidine |
GspSA catalyzes the formation of a peptide bond between GSH and spermidine, cf. EC 6.3.1.8 |
r |
3.5.1.78 | glutathionylspermidine + H2O |
the N-terminal domain catalyzes the hydrolysis of the conjugate |
Escherichia coli |
glutathione + spermidine |
GspSA catalyzes the formation of a peptide bond between GSH and spermidine, cf. EC 6.3.1.8 |
r |
3.5.1.78 | glutathionylspermidine disulfide + H2O |
a coupled reaction of Gsp amidase and glutathione reductase |
Escherichia coli K-12 |
glutathione + ? |
products of the coupled reaction of Gsp amidase and glutathione reductase: spermidine, glutathionylspermidine-glutathione mixed disulfide, and glutathione disulfide are generated |
? |
3.5.1.78 | more |
glutathionylspermidine S-thiolated peptides and proteins are tested as substrates. The protein substrates are treated with disulfide or biotinated glutathionylspermidine to generate biotin-labeled glutathionylspermidine S-thiolated proteins for the detection with an anti-biotin antibody after the reaction with the GspSA. A variety of glutathionylspermidine-derivatized substrates are hydrolyzed yielding spermidine and glutathione S-thiolated protein/peptides |
Escherichia coli K-12 |
? |
- |
? |
3.5.1.78 | more |
bifunctional enzyme that catalyzes the ATP-dependent formation and hydrolysis of glutathionylspermidine, a conjugate of glutathione and spermidine. Proteins YgiC and YjfC, encoded by genes ygiC and yjfC, show ATPase activity, but are not glutathionylspermidine synthetases |
Escherichia coli |
? |
- |
? |
3.5.1.78 | trypanothione + H2O |
- |
Leishmania major |
glutathione + glutathionylspermidine |
- |
? |