EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.1.21.7 | 12-mer oligonucleotide + H2O |
double-stranded, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer |
Tequatrovirus T4 |
hydrolyzed DNA |
- |
? |
| 3.1.21.7 | 34-mer oligonucleotide + H2O |
oligonucleotide duplex containing a fluorine atom at the 2' position of the 5' component of the cyclobutane pyrimidine dimer |
Tequatrovirus T4 |
fragments of 34mer-oligonucleotide |
- |
? |
| 3.1.21.7 | 48-mer oligonucleotide + H2O |
double-stranded, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer |
Tequatrovirus T4 |
24-mer oligonucleotide |
2,3-didehydro-2,3-dideoxyribose 5'-phosphate at the 3'-terminus of the products, initiation of repair synthesis by E. coli DNA-polymerase I |
? |
| 3.1.21.7 | 49-mer oligonucleotide + H2O |
also possesses abasic lyase activity |
Tequatrovirus T4 |
hydrolysed DNA |
- |
? |
| 3.1.21.7 | 49-mer oligonucleotide + H2O |
double-stranded, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer |
Tequatrovirus T4 |
hydrolysed DNA |
- |
? |
| 3.1.21.7 | 49-mer oligonucleotide + H2O |
double-stranded substrate, containing a site-specific duplex cis-syn cyclobutane pyrimidine dimer, enzyme requires a secondary binding event after beta-elimination at a pyrimidine dimer or abasic site |
Tequatrovirus T4 |
3-alpha,beta-unsaturated aldehyde + 3'-phosphate DNA-termini |
delta-elimination reaction |
? |
| 3.1.21.7 | ACUGGACA[rI][rI]U[rI]CUCCGAGG + H2O |
- |
Mus musculus |
? |
- |
? |
| 3.1.21.7 | deoxyinosine-containing deoxyoligonucleotide + H2O |
the enzyme is involved in damaged DNA repair |
Pyrococcus furiosus |
? |
- |
? |
| 3.1.21.7 | deoxyinosine-containing deoxyoligonucleotide + H2O |
the enzyme hydrolyzes the second phosphodiester bond 3' from deoxyinosine |
Pyrococcus furiosus |
? |
- |
? |
| 3.1.21.7 | deoxyinosine-containing RNA + H2O |
(32)P-labeled 21-mer RNA substrate with inosine (5'-CUGUAUGAUGIAGAUGCUGAC-3'). EndoV is a deoxyinosine 3'-endonuclease that recognizes DNA containing deoxyinosine, and cleaves the second and third phosphodiester bonds 3' to the damaged base, leaving a nick with 3' hydroxyl and 5' phosphate groups. Human endonuclease V prefers RNA substrates with inosine over DNA substrates with deoxyinosine |
Homo sapiens |
? |
- |
? |
