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EC Number Substrates Commentary Substrates Organism Products Commentary (Products) Reversibility
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.B1(A)40 + 38 H2O (A)40 is a 40-nt-long synthetic RNA harboring a single radioactively labeled A nucleotide at the 5'-end. When compared with the triphosphorylated RNA, SSSO-RNase J displayes a twofold higher velocity in degrading mono-phosphorylated RNA and RNA bearing a 5'-hydroxyl group Saccharolobus solfataricus adenosine + 39 AMP - ?
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.B1(A)40 + 38 H2O (A)40 is a 40-nt-long synthetic RNA harboring a single radioactively labeled A nucleotide at the 5'-end. When compared with the triphosphorylated RNA, SSSO-RNase J displayes a twofold higher velocity in degrading mono-phosphorylated RNA and RNA bearing a 5'-hydroxyl group Saccharolobus solfataricus P2 adenosine + 39 AMP - ?
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.B15'-pA(A)39 + H2O 5'-pA(A)39 is a 40-nt-long synthetic RNA harboring a 5'-monophosphate and a single radioactively labeled A nucleotide at the 5'-end. When compared with the triphosphorylated RNA, the enzyme displayes a twofold higher velocity in degrading mono-phosphorylated RNA and RNA bearing a 5'-hydroxyl group Saccharolobus solfataricus 40 AMP - ?
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.B15'-pA(A)39 + H2O 5'-pA(A)39 is a 40-nt-long synthetic RNA harboring a 5'-monophosphate and a single radioactively labeled A nucleotide at the 5'-end. When compared with the triphosphorylated RNA, the enzyme displayes a twofold higher velocity in degrading mono-phosphorylated RNA and RNA bearing a 5'-hydroxyl group Saccharolobus solfataricus P2 40 AMP - ?
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.B15'-pC-sR47 + H2O sR47 RNA labeled either at its 5' end as a 5'-[32P]monophosphate. The enzyme is a highly processive 5'-to-3'-exoribonuclease.The principal product of the 5' end-labeled RNA is [32P]GMP as guanosine is the 5'-nucleotide of sR47. Single-stranded DNA is degraded by the enzyme, suggesting that the it does not discriminate between ribose and 2'-deoxyribose nucleosides. Double-stranded DNA is resistant to degradation. The enzyme requires a free 5' end to initiate degradation Pyrococcus abyssi 5'-phosphomononucleotides - ?
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.B15'-pC-sR47 + H2O sR47 RNA labeled either at its 5' end as a 5'-[32P]monophosphate. The enzyme is a highly processive 5'-to-3'-exoribonuclease.The principal product of the 5' end-labeled RNA is [32P]GMP as guanosine is the 5'-nucleotide of sR47. Single-stranded DNA is degraded by the enzyme, suggesting that the it does not discriminate between ribose and 2'-deoxyribose nucleosides. Double-stranded DNA is resistant to degradation. The enzyme requires a free 5' end to initiate degradation Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 5'-phosphomononucleotides - ?
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.B15'-pppA(A)39 + H2O 5'-pppA(A)39 is a 40-nt-long synthetic RNA harboring a 5'-triphosphate and a single radioactively labeled A nucleotide at the 5'-end. When compared with the triphosphorylated RNA, the enzyme displayes a twofold higher velocity in degrading mono-phosphorylated RNA and RNA bearing a 5'-hydroxyl group Saccharolobus solfataricus ATP + 39 AMP - ?
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.B15'-pppA(A)39 + H2O 5'-pppA(A)39 is a 40-nt-long synthetic RNA harboring a 5'-triphosphate and a single radioactively labeled A nucleotide at the 5'-end. When compared with the triphosphorylated RNA, the enzyme displayes a twofold higher velocity in degrading mono-phosphorylated RNA and RNA bearing a 5'-hydroxyl group Saccharolobus solfataricus P2 ATP + 39 AMP - ?
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.B15'-pppA(A)41 + H2O 5'-pppA(A)41 is a 42-nt-long synthetic RNA harboring a 5'-tri-phosphate and a single radioactively labeled A nucleotide at the 5'-end. Decay of 5'-PPP-40A1 RNA results in either a single nucleotide or short oligoribonucleotides, indicating an exoribonucleolytic activity. Degradation with 5' to 3' directionality in vitro Sulfolobus acidocaldarius ATP + 41 AMP - ?
Display the word mapDisplay the reaction diagram Show all sequences 3.1.13.B15'-pppA(A)41 + H2O 5'-pppA(A)41 is a 42-nt-long synthetic RNA harboring a 5'-tri-phosphate and a single radioactively labeled A nucleotide at the 5'-end. Decay of 5'-PPP-40A1 RNA results in either a single nucleotide or short oligoribonucleotides, indicating an exoribonucleolytic activity. Degradation with 5' to 3' directionality in vitro Sulfolobus acidocaldarius DSM 639 ATP + 41 AMP - ?
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