EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.1.11.2 | apurinic DNA + H2O |
- |
Escherichia coli |
? |
- |
? |
| 3.1.11.2 | DNA + H2O |
the enzyme digests blunt-ended dsDNA successively. ssDNA remains almost completely intact. 3'-recessed dsDNA is digested in the same manner as blunt-ended dsDNA. 3'-protruding ended dsDNA shows resistance to degradation. The enzyme exhibits dsDNA-specific exonuclease activity from the 3' to 5' direction. A nick site in dsDNA and dsDNA with 3'-phosphate termini are also susceptible to digestion. The enzyme can exhibit exonuclease activity from a nick and possesses 3'-phosphomonoesterase activity. The limited length of the product is observed to be 11 nt. the enzyme may require at least 11-nt DNA for binding |
Methanosarcina acetivorans |
? |
- |
? |
| 3.1.11.2 | DNA + H2O |
the enzyme digests blunt-ended dsDNA successively. ssDNA remains almost completely intact. 3'-recessed dsDNA is digested in the same manner as blunt-ended dsDNA. 3'-protruding ended dsDNA shows resistance to degradation. The enzyme exhibits dsDNA-specific exonuclease activity from the 3' to 5' direction. A nick site in dsDNA and dsDNA with 3'-phosphate termini are also susceptible to digestion. The enzyme can exhibit exonuclease activity from a nick and possesses 3'-phosphomonoesterase activity. The limited length of the product is observed to be 11 nt. the enzyme may require at least 11-nt DNA for binding |
Methanosarcina acetivorans ATCC 35395 |
? |
- |
? |
| 3.1.11.2 | DNA duplex X1 + H2O |
MtbXthA possesses a 3'-5' exonuclease activity on 3' recessed duplex DNA, substrate is 5'-FAM peptide-labelled 3'-recessed DNA duplex substrate X1. The incubation of substrate X1 with restrictive concentration of purified MtbXthAalone resulted in only 11% cleavage generating shorter-labelled oligomers. The inclusion of beta-clamp in the reaction mixture substantially enhances the exonuclease activity |
Mycobacterium tuberculosis |
? |
- |
? |
| 3.1.11.2 | DNA duplex X1 + H2O |
MtbXthA possesses a 3'-5' exonuclease activity on 3' recessed duplex DNA, substrate is 5'-FAM peptide-labelled 3'-recessed DNA duplex substrate X1. The incubation of substrate X1 with restrictive concentration of purified MtbXthAalone resulted in only 11% cleavage generating shorter-labelled oligomers. The inclusion of beta-clamp in the reaction mixture substantially enhances the exonuclease activity |
Mycobacterium tuberculosis H37Rv |
? |
- |
? |
| 3.1.11.2 | DNA duplex X1 + H2O |
MtbXthA possesses a 3'-5' exonuclease activity on 3' recessed duplex DNA, substrate is 5'-FAM peptide-labelled 3'-recessed DNA duplex substrate X1. The incubation of substrate X1 with restrictive concentration of purified MtbXthAalone resulted in only 11% cleavage generating shorter-labelled oligomers. The inclusion of beta-clamp in the reaction mixture substantially enhances the exonuclease activity |
Mycobacterium tuberculosis ATCC 25618 |
? |
- |
? |
| 3.1.11.2 | double-stranded DNA + H2O |
- |
Escherichia coli |
more |
- |
? |
| 3.1.11.2 | double-stranded DNA + H2O |
- |
Escherichia coli |
more |
the size of the products after 3'-5' hydrolysis is temperature-dependent. At 25°C the enzyme can hydrolyze a polynucleotide chain up to the last 5'-terminal dinucleotide. A gradation of higher 5'-terminal oligonucleotides of defined chain lengths is produced after limit digestion by the enzyme when the temperature is raised from 25°C to 60°C |
? |
| 3.1.11.2 | double-stranded DNA + H2O |
3'-phosphoryl-terminated DNA |
Escherichia coli |
more |
5'-mononucleotides + phosphate + large molecular weight single stranded oligonucleotides |
? |
| 3.1.11.2 | double-stranded DNA + H2O |
sonicated T7 DNA |
Escherichia coli |
more |
- |
? |
