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Results 1 - 10 of 16 > >>
EC Number Substrates Commentary Substrates Organism Products Commentary (Products) Reversibility
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22more formation of a Hermes transposase-DNA complex. Bipartite DNA recognition at hAT transposon ends and Hermes-DNA interactions within the transpososome. No protein-DNA interactions involving bp 12-16 of the TIR. The avidity provided by multiple sites of interaction allows a transposase to locate its transposon ends amidst a sea of chromosomal DNA, mechanism, overview. The enzyme possesses a RNaseH-like catalytic domain interrupted by a large [.alpha]-helical insertion domain, and an N-terminal intertwined dimerization domain. Together, these domains catalyze the chemical steps of DNA nicking, hairpin formation, and DNA strand transfer that comprise hAT transposition Musca domestica ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22more H2K A/J muscle cells properly express full-length human DYSF following SB-mediated gene transfer. A duplicate of Spc5-12 (2xSpc5-12) regulatory sequence proves to be the most efficient in driving transgene expression in H2K A/J myoblasts. Corrected H2K A/J myoblasts can efficiently be transplanted into Scid/BLA/J mouse muscle Homo sapiens ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22more the sleeping beauty transposon DNA contains the gene to be inserted into the target DNA flanked by inverted terminal repeats (IR-DRleft and IR-DRright). Each inverted terminal repeat contains two, inner and outer, direct repeats (DRs). The DRs represent binding sites for the transposase enzyme. Sleeping beauty, SB, appears to be the most random in preferences for integration sites, requiring only a TA dinucleotide basepair. Binding mechanism, detailed overview Homo sapiens ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22more for examination of the transposition activity of recombinant Tgf2 TPase, microinjection is performed in blunt snout bream embryos at 1-2 cell stage. When donor plasmid pTgf2-EF1alpha-EGFP is co-injected with recombinant Tgf2 TPase protein, 74% of the embryos show almost ubiquitous and uniform expression of EGFP, while 31% control embryos by injected donor plasmid show only mosaic expression of EGFP. Four EGFP-positive blunt snout bream are sampled from the group coinjected with donor plasmid with recombinant Tgf2 TPase. An 8-bp direct repeat of target DNA at the integration site, the signature of hAT family transposons, is created adjacent to both ends of Tgf2 at the integration sites in all fish, indicating the recombinant Tgf2 TPase insertions occur by transposition Carassius auratus ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22more identification of multiple DNA binding sites for the THAP domain of the Galileo transposase in the long terminal inverted-repeats, these TIRs and other Foldback-like elements may provide the transposase with its binding specificity Drosophila buzzatii ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22more interaction analysis of full-length enzyme and of PAI subdomain with transposon DNA, overview Homo sapiens ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22more Sleeping Beauty transposase excision activity is quantified using a fluorescence-activated cell sorting (FACS)-based excision assay in HeLa cells, overall SB transposition activity is also detected in HeLa cells Homo sapiens ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22more Tgf2 is an autonomously active vertebrate transposon that is efficient at gene-transfer in teleost fish. The N-terminal zinc finger domain of Tgf2 transposase contributes to DNA binding and to transposition activity. Proposed model for Tgf2 transposition, overview Carassius auratus ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22more The reaction of DNA transposition begins when the transposase enzyme binds to the transposon DNA. Folding of the specific DNA recognition subdomain of the sleeping beauty transposase is temperature-dependent and is required for its binding to the transposon DNA. Only the folded conformation of the specific DNA recognition subdomain of the Sleeping Beauty transposase, the PAI subdomain, binds to the transposon DNA. The PAI subdomain is well folded at low temperatures, but the presence of unfolded conformation gradually increases at temperatures above 15°C. DNA-binding of folded and unfolded conformations of the PAI subdomain, overview Homo sapiens ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22more the TnpA transposase precisely cleaves leaft and right ends (LE and RE) of a gene without leaving behind a scar sequence. Development of a simple and precise method for genome manipulation in Escherichia coli that alters the gene sequence without leaving foreign DNA in the chromosome. This strategy involves PCR amplification of a DNA cassette containing ISHp608-LE (left end)-antibiotic resistance gene-counterselection marker-ISHp608-RE (right end) by using primers containing extensions homologous to the adjacent regions of the target gene on the chromosome. The lambda Red-mediated recombination of the PCR product and antibiotic resistance screening results in transformants with a modified gene target. The ISHp608-LE-antibiotic resistance gene-counterselection marker-ISHp608-RE cassette can then be excised using a temperature sensitive plasmid expressing the TnpA transposase, which precisely cleaves ISHp608-LE and ISHp608-RE without leaving a scar sequence. For introduction of IS608 LE and RE into the gene of interest, lambda-Red recombination is utilized, which does not require in vitro manipulations such as restriction digestion, ligation or construction of a suicide vector. Diagram of plasmids containing selectable and excisable IS608 cassettes, overview Escherichia coli O103:H2 ? - ?
Results 1 - 10 of 16 > >>