EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   2.7.7.7 | 2'-deoxyguanosine triphosphate + DNAn |
- |
Drosophila melanogaster |
diphosphate + DNAn+1 |
- |
? |
| 2.7.7.7 | 2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate + DNAn |
- |
Saccharolobus solfataricus |
diphosphate + ? |
- |
? |
| 2.7.7.7 | 2-aminopurine-2'-deoxy-D-ribose 5'-triphosphate + DNAn |
- |
Saccharolobus solfataricus P2 |
diphosphate + ? |
- |
? |
| 2.7.7.7 | 2-hydroxy-2'-deoxyadenosine 5'-triphosphate + DNAn |
DNA polymerase eta incorporates 2-hydroxy-2'-deoxyadenosine 5'-triphosphate opposite template G during DNA synthesis |
Homo sapiens |
diphosphate + DNAn+1 |
- |
? |
| 2.7.7.7 | 2-thio-dCTP + DNAn |
dCTP and 5-methyl-dCTP are efficiently incorporated opposite a template guanine but significantly less so opposite a template O6-methylguanine. 2-thio-dCTP is efficiently inserted opposite guanine and is also incorporated opposite O6-methylguanine, to a similar extent as dCTP. Of the dNTPs assayed, dCTP, 5-Me-dCTP, and 2-thio-dCTP display the highest incorporation efficiency opposite O6-methylguanine. dTTP incorporation is favored opposite O6-methylguanine rather than opposite guanine. Hydrophobicity of the incoming dNTP appears to have little influence on the process of nucleotide selection by Dpo4, with hydrogen bonding capacity being a major influence. 8-oxo-dATP and 8-bromo-dATP are not inserted opposite O6-methylguanine and are slowly incorporated opposite guanine. dPTP (i.e. 6H,8H-3,4-dihydro-pyrimido[4,5-c][1,2]oxazin-7-one-8-b-d-2-deoxyribofuranosid-5-triphosphate) is incorporated opposite guanine slightly less efficiently than dCTP and is not incorporated opposite O6-methylguanine |
Saccharolobus solfataricus |
? |
- |
? |
| 2.7.7.7 | 2-thio-dCTP + DNAn |
dCTP and 5-methyl-dCTP are efficiently incorporated opposite a template guanine but significantly less so opposite a template O6-methylguanine. 2-thio-dCTP is efficiently inserted opposite guanine and is also incorporated opposite O6-methylguanine, to a similar extent as dCTP. Of the dNTPs assayed, dCTP, 5-Me-dCTP, and 2-thio-dCTP display the highest incorporation efficiency opposite O6-methylguanine. dTTP incorporation is favored opposite O6-methylguanine rather than opposite guanine. Hydrophobicity of the incoming dNTP appears to have little influence on the process of nucleotide selection by Dpo4, with hydrogen bonding capacity being a major influence. 8-oxo-dATP and 8-bromo-dATP are not inserted opposite O6-methylguanine and are slowly incorporated opposite guanine. dPTP (i.e. 6H,8H-3,4-dihydro-pyrimido[4,5-c][1,2]oxazin-7-one-8-b-d-2-deoxyribofuranosid-5-triphosphate) is incorporated opposite guanine slightly less efficiently than dCTP and is not incorporated opposite O6-methylguanine |
Saccharolobus solfataricus P2 |
? |
- |
? |
| 2.7.7.7 | 5-ethynyl-dCTP + DNAn |
- |
Geobacillus stearothermophilus |
diphosphate + DNAn+1 |
- |
? |
| 2.7.7.7 | 5-ethynyl-dUTP + DNAn |
- |
Geobacillus stearothermophilus |
diphosphate + DNAn+1 |
- |
? |
| 2.7.7.7 | 5-methyl-dCTP + DNAn |
dCTP and 5-methyl-dCTP are efficiently incorporated opposite a template guanine but significantly less so opposite a template O6-methylguanine. 2-thio-dCTP is efficiently inserted opposite guanine and is also incorporated opposite O6-methylguanine, to a similar extent as dCTP. Of the dNTPs assayed, dCTP, 5-Me-dCTP, and 2-thio-dCTP display the highest incorporation efficiency opposite O6-methylguanine. dTTP incorporation is favored opposite O6-methylguanine rather than opposite guanine. Hydrophobicity of the incoming dNTP appears to have little influence on the process of nucleotide selection by Dpo4, with hydrogen bonding capacity being a major influence. 8-oxo-dATP and 8-bromo-dATP are not inserted opposite O6-methylguanine and are slowly incorporated opposite guanine. dPTP (i.e. 6H,8H-3,4-dihydro-pyrimido[4,5-c][1,2]oxazin-7-one-8-b-d-2-deoxyribofuranosid-5-triphosphate) is incorporated opposite guanine slightly less efficiently than dCTP and is not incorporated opposite O6-methylguanine |
Saccharolobus solfataricus |
? |
- |
? |
| 2.7.7.7 | 5-methyl-dCTP + DNAn |
dCTP and 5-methyl-dCTP are efficiently incorporated opposite a template guanine but significantly less so opposite a template O6-methylguanine. 2-thio-dCTP is efficiently inserted opposite guanine and is also incorporated opposite O6-methylguanine, to a similar extent as dCTP. Of the dNTPs assayed, dCTP, 5-Me-dCTP, and 2-thio-dCTP display the highest incorporation efficiency opposite O6-methylguanine. dTTP incorporation is favored opposite O6-methylguanine rather than opposite guanine. Hydrophobicity of the incoming dNTP appears to have little influence on the process of nucleotide selection by Dpo4, with hydrogen bonding capacity being a major influence. 8-oxo-dATP and 8-bromo-dATP are not inserted opposite O6-methylguanine and are slowly incorporated opposite guanine. dPTP (i.e. 6H,8H-3,4-dihydro-pyrimido[4,5-c][1,2]oxazin-7-one-8-b-d-2-deoxyribofuranosid-5-triphosphate) is incorporated opposite guanine slightly less efficiently than dCTP and is not incorporated opposite O6-methylguanine |
Saccharolobus solfataricus P2 |
? |
- |
? |
