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EC Number Substrates Commentary Substrates Organism Products Commentary (Products) Reversibility
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.1025'-CTTCTTCTGTGC-3' + n dNTP i.e. minimal substrate which supports synthesis of the full-length primer. The base 3' to the central GTG motif is critical for primer synthesis and the bases 5' of the GTG determine the length of the primer or run-off product Sulfolobus islandicus 5'-CTTCTTCTGTGC-3'/pppdN(pdN)n-1 + (n-1) diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.1025'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3' + n dNTP - Saccharolobus solfataricus 5'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3'/pppdN(pdN)n-1 + n-1 diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.1025'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3' + n dNTP - Saccharolobus solfataricus DSM 1617 5'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3'/pppdN(pdN)n-1 + n-1 diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.1025'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3' + n NTP - Saccharolobus solfataricus 5'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3'/pppN(dN)n-1 + n-1 diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.1025'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3' + n NTP - Saccharolobus solfataricus DSM 1617 5'-FAM-AAAAAAAAAAATCAGCGGACAAAAAAAAAAAA-3'/pppN(dN)n-1 + n-1 diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.1025'-TTTTTTTTGTGCACTTT + n dNTP - Sulfolobus islandicus 5'-TTTTTTTTGTGCACTTT/pppdN(pdN)n-1 + (n-1) diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.102ATP + 7 dNTP enzyme synthesizes short (8 nt-long) primers in the presence of single-stranded M13 DNA. The 12-bases-long substrate with the sequence 5'-CTTCTTCTGTGC-3' represents the minimal substrate which supports synthesis of the full-length primer. The base 3' to the central GTG motif is critical for primer synthesis and that the bases 5' of the GTG determine the length of the primer or run-off product but do not appear to be important for the efficiency of primer synthesis Sulfolobus islandicus A(pdN)7 + 7 diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.102ATP + 7 dNTP formation of a ribonucleotide primer when ORF904 is incubated with ribonucleotides and single-stranded M13 DNA. Effcient dNTP incorporation is strongly dependent on the presence of ATP. The non-hydrolysable analogues beta,gamma-imino-ATP and beta,gamma-methylene-ATP are active in stimulation of primer formation. alpha,beta-methylene-ATP does not enhance primer formation. The stimulatory effect is also seen for GTP and UTP but not for ADP or dATP. The majority of the primers have a length of eight nucleotides. Protein ORF904 shows ATPase, DNA polymerase and primase activity Sulfolobus islandicus A(pdN)7 + 7 diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.102bacteriophage M13 ssDNA + n NTP - Saccharolobus solfataricus bacteriophage M13 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.102bacteriophage M13 ssDNA + n NTP - Saccharolobus solfataricus ATCC 35092 bacteriophage M13 ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate - ?
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