2.7.2.15 | more |
enzyme binding of nucleotide mainly involves hydrophobic interactions with the base moiety and can account for the broad biochemical specificity observed between the enzyme and nucleotides, modeling. Residue Ala88 is an important residue involved in determining the rate of catalysis with short-chain fatty acid substrates. No activity with with several closely related analogues such as formate, isobutyrate, 2-oxobutyrate, malic acid, citrate, isocitric acid, oxaloacetate, succinate and 2-oxoglutarate, and also no activit with short-chain alcohols methanol, ethanol, propanol, and butanol, and aldehydes acetaldehyde and propionaldehyde. An ethylene glycol molecule bound sufficiently close to the gamma-phosphate in StTdcD complexes with triphosphate nucleotides supports direct in-line phosphoryl transfer |
Salmonella enterica subsp. enterica serovar Typhimurium |
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