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Results 1 - 5 of 5
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EC Number Substrates Commentary Substrates Organism Products Commentary (Products) Reversibility
Display the reaction diagram Show all sequences 2.5.1.97more N-acetyl-D-mannosamine can not replace 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose as a substrate indicating that the enzyme does not possess detectable sialic acid synthase activity Campylobacter jejuni ? - ?
Display the reaction diagram Show all sequences 2.5.1.97phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O pseudaminic acid biosynthesis Helicobacter pylori phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid - ?
Display the reaction diagram Show all sequences 2.5.1.97phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O pseudaminic acid biosynthesis Campylobacter jejuni phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid - ?
Display the reaction diagram Show all sequences 2.5.1.97phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O combining the five enzymes of pseudaminic acid biosynthesis (PseB, PseC, PseH, PseG, PseI) in a one-pot reaction results in 100% conversion of UDP-N-acetylglucosamine to pseudaminic acid after 20 h Helicobacter pylori phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid - ?
Display the reaction diagram Show all sequences 2.5.1.97phosphoenolpyruvate + 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose + H2O phosphoenolpyruvate attacks the si-face of the aldehyde of 2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose to generate an (S)-configuration at C-4. The proposed catalytic mechanism of pseudaminic acid synthase is the attack of the C-3 of PEP to the aldehyde of the open chain form of 6-deoxy-AltdiNAc and an attack of water to the C-2 of phosphoenolpyruvate to form a tetrahedral intermediate. The tetrahedral intermediate then collapses to release inorganic phosphate and form the open chain form of pseudaminic acid that cyclizes to the pyranose form in solution Campylobacter jejuni phosphate + 5,7-bis(acetylamino)-3,5,7,9-tetradeoxy-L-glycero-alpha-L-manno-2-nonulopyranosonic acid - ?
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