EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.4.2.48 | guanine15 in tRNA + 7-cyano-7-carbaguanine |
key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs |
Haloferax volcanii |
7-cyano-7-carbaguanine15 in tRNA + guanine |
- |
? |
2.4.2.48 | guanine15 in tRNA + 7-cyano-7-carbaguanine |
archaeosine base and 7-aminomethyl-7-deazaguanine are not incorporated into tRNA by this enzyme. Does not require ATP for the base replacement reaction |
Haloferax volcanii |
7-cyano-7-carbaguanine15 in tRNA + guanine |
- |
? |
2.4.2.48 | guanine15 in tRNA + 7-cyano-7-carbaguanine |
key enzyme for the biosynthetic pathway leading to archaeosine in archaeal tRNAs |
Haloferax volcanii ATCC 29605 |
7-cyano-7-carbaguanine15 in tRNA + guanine |
- |
? |
2.4.2.48 | guanine15 in tRNA + 7-cyano-7-carbaguanine |
archaeosine base and 7-aminomethyl-7-deazaguanine are not incorporated into tRNA by this enzyme. Does not require ATP for the base replacement reaction |
Haloferax volcanii ATCC 29605 |
7-cyano-7-carbaguanine15 in tRNA + guanine |
- |
? |
2.4.2.48 | guanine15 in tRNA + 7-cyano-7-carbaguanine |
i.e. 7-(cyano)-7-deazaguanine = preQ0 |
Methanocaldococcus jannaschii |
7-cyano-7-carbaguanine15 in tRNA + guanine |
- |
? |
2.4.2.48 | guanine15 in tRNA + 7-cyano-7-carbaguanine |
i.e. 7-(cyano)-7-deazaguanine = preQ0. Haloferax volcanii tRNASer is used as substrate |
Methanocaldococcus jannaschii |
7-cyano-7-carbaguanine15 in tRNA + guanine |
- |
? |
2.4.2.48 | more |
the enzyme catalyzes a transglycosylation reaction in which guanine is eliminated from position 15 of the tRNA and an archaeosine precursor (preQ0) is inserted. The enzyme is able to utilize both guanine and the 7-deazaguanine base preQ0 as substrates, but not other 7-deazaguanine bases, and is able to modify tRNA from all three phylogenetic domains. The enzyme shows good activity with the tRNASer transcript as well as tRNA from Escherichia coli and yeast |
Methanocaldococcus jannaschii |
? |
- |
? |
2.4.2.48 | guanine15 in tRNA + 7-cyano-7-carbaguanine |
- |
Methanosarcina acetivorans |
7-cyano-7-carbaguanine15 in tRNA + guanine |
- |
? |
2.4.2.48 | guanine15 in tRNA + 7-cyano-7-deazaguanine |
- |
Methanosarcina acetivorans |
7-cyano-7-deazaguanine15 in tRNA + guanine |
- |
? |
2.4.2.48 | guanine15 in tRNA + 7-cyano-7-carbaguanine |
- |
Methanosarcina acetivorans DSM 2834 |
7-cyano-7-carbaguanine15 in tRNA + guanine |
- |
? |