EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.3.1.94 | 6 methylmalonyl-CoA + propionyl-CoA + 6 NADPH + 6 H+ |
- |
Saccharopolyspora erythraea |
6-deoxyerythronolide b + 7 CoA + 6 CO2 + H2O + 6 NADP+ |
- |
? |
2.3.1.94 | 6 methylmalonyl-CoA + propionyl-CoA + 6 NADPH + 6 H+ |
formation of a key intermediate in the biosynthesis of erythromycin |
Saccharopolyspora erythraea |
6-deoxyerythronolide b + 7 CoA + 6 CO2 + H2O + 6 NADP+ |
- |
? |
2.3.1.94 | more |
unidirectional translocation of the growing polyketide chain along the 6-deoxyerythronolide B synthase, ratchet mechanism, overview |
Saccharopolyspora erythraea |
? |
- |
? |
2.3.1.94 | more |
the cis-acyltransferase domain from DEBS module 3 transfers a methylmalonyl extender unit from the corresponding carboxyacyl-CoA precursor onto the phosphopantetheine arm of the acyl carrier protein within the same module. The presence of the acyl carrier protein has little effect on the specificity of the cis-acyltransferase domain for carboxyacyl-CoA substrates, but has a marked influence on the corresponding specificity parameters for the trans-acyltransferases from the disorazole and kirromycin synthases. Comparative analysis of the substrate specificity |
Saccharopolyspora erythraea |
? |
- |
? |
2.3.1.94 | propanoyl-CoA + (2S)-methylmalonyl-CoA + NADPH |
- |
Saccharopolyspora erythraea |
(3R,4S,5R,6R)-6-ethyl-4-hydroxy-3,5-dimethyloxan-2-one + ? |
- |
? |
2.3.1.94 | propanoyl-CoA + 5 (2S)-methylmalonyl-CoA + (2S)-ethylmalonyl-CoA + 6 NADPH + 6 H+ |
- |
Saccharopolyspora erythraea |
(3R,4S,5R,6R)-6-ethyl-4-hydroxy-3,5-dimethyltetrahydro-2H-pyran-2-one + ? |
product of truncated enzyme consisting of loading didomain-module1-module2+thioesterase |
? |
2.3.1.94 | propanoyl-CoA + 5 (2S)-methylmalonyl-CoA + (2S)-ethylmalonyl-CoA + 6 NADPH + 6 H+ |
- |
Saccharopolyspora erythraea |
(3R,5R,6S)-6-[(2S,3R)-3-hydroxypentan-2-yl]-3,5-dimethyldihydro-2H-pyran-2,4(3H)-dione + ? |
product of truncated enzyme consisting of loading didomain-module1-module2-module3+thioesterase |
? |
2.3.1.94 | propanoyl-CoA + 5 (2S)-methylmalonyl-CoA + (2S)-ethylmalonyl-CoA + 6 NADPH + 6 H+ |
- |
Saccharopolyspora erythraea |
8-ethyl-8-desmethyl-6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+ |
putative analog, 8-ethyl-8-desmethyl-6-deoxyerythronolide B, is generated by the assembly line in the presence of non-limiting concentrations of ethylmalonyl-CoA and is produced in comparable amounts to the natural 6-dEB product |
? |
2.3.1.94 | propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH + 6 H+ |
- |
Saccharopolyspora erythraea |
6-deoxyerythronolide B + 7 CoA + 6 CO2 + H2O + 6 NADP+ |
- |
? |