EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   2.1.1.34 | more |
transfers a methyl group to the 2'-hydroxy group of a guanosine residue of a GG-sequence in methyl-deficient tRNATyr from E. coli 58-161 |
Escherichia coli |
? |
- |
? |
| 2.1.1.34 | more |
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli |
Thermus thermophilus |
? |
- |
? |
| 2.1.1.34 | more |
enzyme hydroxylates guanosine at postion 18 in tRNA |
Thermus thermophilus |
? |
- |
? |
| 2.1.1.34 | more |
recognition region is included within the D-arm structure of tRNA-Leu, a bulge is essentially required. 4 base pairs within the D-stem enhance methyl acceptance activity. Py16Py17G18G19 sequence is important for efficient methyl tranfer |
Aquifex aeolicus |
? |
- |
? |
| 2.1.1.34 | more |
yeast tRNAPhe mutant transcripts are prepared in which the modification site and/or other nucleotides in the D-loop are substituted by dG, inosine, or other nucleotides. Sites of methylation are confirmed with RNA sequencing or primer extension. Although the G18G19 sequence is not essential for methylation by TrmH, disruption of G18G19 severely reduces the efficiency of methyl transfer. There is strict recognition of guanosine by TrmH, in that methylation occurs at the adjacent G19 when the G18 is replaced by dG or adenosine. The fact that TrmH methylates guanosine in D-loops from 4 to 12 nucleotides in length suggests that selection of the position of guanosine within the D-loop is relatively flexible |
Thermus thermophilus |
? |
- |
? |
| 2.1.1.34 | more |
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 |
? |
- |
? |
| 2.1.1.34 | more |
specifically methylates guanosine at position 18 in the D-loop of tRNAPhe from E. coli |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 |
? |
- |
? |
| 2.1.1.34 | more |
transfers a methyl group to the 2'-hydroxy group of a guanosine residue of a GG-sequence in methyl-deficient tRNATyr from E. coli 58-161 |
Escherichia coli CA274 |
? |
- |
? |
| 2.1.1.34 | S-adenosyl-L-methionine + Escherichia coli tRNA-Met |
- |
Thermus thermophilus |
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Met |
- |
? |
| 2.1.1.34 | S-adenosyl-L-methionine + guanosine18 in Escherichia coli tRNA-Ile |
- |
Thermus thermophilus |
S-adenosyl-L-homocysteine + 2'-O-methylguanosine18 in Escherichia coli tRNA-Ile |
- |
? |
