EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.283 | more |
the enzyme shows a broad substrate specificity. SpOMT7740 catalyzes the O-methylation reaction to form various natural and non-natural O-methoxides, includes 7-hydroxy-8-O-methoxy flavone, 3-O-methoxy flavone, three mono-, di-, and tri-O-methoxy genistein, mono-O-methoxy phloretin, mono-O-methoxy luteolin, 3-O-methoxy beta-sitosterol, and O-methoxy anthraquinones (emodin and aloe emodin) and O-methoxy anthracycline (daunorubicin) exhibiting diverse substrate flexibility. Mass spectrometric analysis of reaction products. 7,8-Dihydroxyflavone is the best substrate for bioconversion to 7-hydroxy-8-O-methoxy flavone. The enzyme seems to prefer methylation of hydroxy groups at C8 position. No or poor activity with doxorubicin and daunorubicin |
Streptomyces peucetius subsp. caesius |
? |
- |
- |
2.1.1.283 | S-adenosyl-L-methionine + 1,3,8-trihydroxy-6-methoxy-9,10-anthraquinone |
i.e. citreorosein, 22% of the activity compared to emodin |
Aspergillus terreus |
? |
- |
? |
2.1.1.283 | S-adenosyl-L-methionine + 1,3,8-trihydroxy-6-methoxy-9,10-anthraquinone |
i.e. citreorosein, 22% of the activity compared to emodin |
Aspergillus terreus IMI 16043 |
? |
- |
? |
2.1.1.283 | S-adenosyl-L-methionine + 3,5,7,8,3',4'-hexahydroxyflavone |
- |
Streptomyces peucetius subsp. caesius |
S-adenosyl-L-homocysteine + 3,5,7,3',4'-pentahydroxy-8-methoxyflavone |
- |
? |
2.1.1.283 | S-adenosyl-L-methionine + 3-hydroxyflavone |
lower activity |
Streptomyces peucetius subsp. caesius |
S-adenosyl-L-homocysteine + 3-methoxyflavone |
- |
? |
2.1.1.283 | S-adenosyl-L-methionine + 7,8-dihydroxyflavone |
best substrate |
Streptomyces peucetius subsp. caesius |
S-adenosyl-L-homocysteine + 7-hydroxy-8-methoxyflavone |
- |
? |
2.1.1.283 | S-adenosyl-L-methionine + 7-chloroemodin |
18% of the activity compared to emodin |
Aspergillus terreus |
? |
- |
? |
2.1.1.283 | S-adenosyl-L-methionine + 7-chloroemodin |
18% of the activity compared to emodin |
Aspergillus terreus IMI 16043 |
? |
- |
? |
2.1.1.283 | S-adenosyl-L-methionine + aloe emodin |
low activity |
Streptomyces peucetius subsp. caesius |
S-adenosyl-L-homocysteine + 8-methoxy-1-hydroxy-3-(hydroxymethyl)anthracene-9,10-dione |
- |
? |
2.1.1.283 | S-adenosyl-L-methionine + beta-sitosterol |
low activity |
Streptomyces peucetius subsp. caesius |
S-adenosyl-L-homocysteine + 3-methoxy-beta-sitosterol |
- |
? |