EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
  2.1.1.266 | more |
enzyme activity with in vitro modified potential YhiR substrates prepared from the enzyme-deficient DELTAyhiR strain: methylation of the 50S subunits, NH4Cl/ethanol split particles, LiCl split particles, and deproteinized 23S rRNA, overview |
Escherichia coli |
? |
- |
? |
| 2.1.1.266 | S-adenosyl-L-methionine + adenine2030 in 23S rRNA |
- |
Escherichia coli |
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA |
- |
? |
| 2.1.1.266 | S-adenosyl-L-methionine + adenine2030 in 23S rRNA |
nascent 23S rRNA is the natural substrate for YhiR |
Escherichia coli |
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA |
- |
? |
| 2.1.1.266 | S-adenosyl-L-methionine + adenine2030 in 23S rRNA |
adenine-N6 methyltransferase, encoded by gene yhiR, is specific for modification of A2030 of 23S ribosomal RNA. YhiR by itself, without any additional proteins, is able to modify 23S rRNA |
Escherichia coli |
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA |
mass spectrometric product identification, overview |
? |
| 2.1.1.266 | S-adenosyl-L-methionine + adenine2030 in 23S rRNA |
enzyme RlmJ uses the cofactor S-adenosyl-L-methionine to methylate the exocyclic nitrogen N6 of nucleotide A2030 in 23S rRNA during ribosome assembly in Escherichia coli |
Escherichia coli |
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA |
- |
? |
| 2.1.1.266 | S-adenosyl-L-methionine + adenine2030 in 23S rRNA |
In vitro transcription of 23S rRNA substrate from the wild-type BW25113 strain. RlmJ methylates in vitro transcribed 23S rRNA, as well as a minimal substrate corresponding to helix 72, demonstrating independence of modifications and tertiary interactions in the RNA substrate. RlmJ displays specificity for adenosine, substrate binding site structure, overview. Location of A2030 in Escherichia coli 23S rRNA, overview |
Escherichia coli |
S-adenosyl-L-homocysteine + N6-methyladenine2030 in 23S rRNA |
- |
? |
