EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.217 | more |
the methyltransferase TrmK (BsTrmK) is responsible for the formation of m1A22 in tRNA. BsTrmK displays a broad substrate specificity, and methylates seven out of eight tRNA isoacceptor families of Bacillus subtilis bearing an A22. In addition to a non-Watson-Crick base-pair between the target A22 and a purine at position 13, the formation of m1A22 by BsTrmK requires a full-length tRNA with intact tRNA elbow and anticodon stem. Measurements of the MTase activity using 32P-radiolabelled Bacillus subtilis tRNAs. Interaction between BsTrmK and the cofactor product S-adenosyl-L-homocysteine (SAH) is enthalpy-driven with a single binding site and a dissociation constant (KD) of 0.0017 mM, residues R9, L10, G77, D78, A94 and G95 are involved in SAH binding |
Bacillus subtilis |
? |
- |
- |
2.1.1.217 | more |
the methyltransferase TrmK (BsTrmK) is responsible for the formation of m1A22 in tRNA. BsTrmK displays a broad substrate specificity, and methylates seven out of eight tRNA isoacceptor families of Bacillus subtilis bearing an A22. In addition to a non-Watson-Crick base-pair between the target A22 and a purine at position 13, the formation of m1A22 by BsTrmK requires a full-length tRNA with intact tRNA elbow and anticodon stem. Measurements of the MTase activity using 32P-radiolabelled Bacillus subtilis tRNAs. Interaction between BsTrmK and the cofactor product S-adenosyl-L-homocysteine (SAH) is enthalpy-driven with a single binding site and a dissociation constant (KD) of 0.0017 mM, residues R9, L10, G77, D78, A94 and G95 are involved in SAH binding |
Bacillus subtilis 168 |
? |
- |
- |
2.1.1.217 | S-adenosyl-L-methionine + adenine22 in Bacillus subtilis tRNA(Ser) |
crude Escherichia coli tRNA is used, since it does not contain m1A22 |
Bacillus subtilis |
S-adenosyl-L-homocysteine + N1-methyladenine22 in Bacillus subtilis tRNA(Ser) |
- |
? |
2.1.1.217 | S-adenosyl-L-methionine + adenine22 in tRNA |
- |
Streptococcus pneumoniae |
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA |
- |
? |
2.1.1.217 | S-adenosyl-L-methionine + adenine22 in tRNA |
- |
Bacillus subtilis |
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA |
- |
? |
2.1.1.217 | S-adenosyl-L-methionine + adenine22 in tRNA |
- |
Vibrio cholerae |
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA |
- |
? |
2.1.1.217 | S-adenosyl-L-methionine + adenine22 in tRNA |
- |
Listeria monocytogenes serotype 4b |
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA |
- |
? |
2.1.1.217 | S-adenosyl-L-methionine + adenine22 in tRNA |
- |
Staphylococcus aureus |
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA |
- |
? |
2.1.1.217 | S-adenosyl-L-methionine + adenine22 in tRNA |
a methyl group at position N1 prevents Watson-Crick-type base pairing by adenosine and is therefore important for regulation of structure and stability of tRNA molecules |
Bacillus subtilis |
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA |
- |
? |
2.1.1.217 | S-adenosyl-L-methionine + adenine22 in tRNA |
crude Escherichia coli tRNA is used, since it does not contain m1A22 |
Bacillus subtilis |
S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA |
- |
? |