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Results 1 - 5 of 5
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EC Number Substrates Commentary Substrates Organism Products Commentary (Products) Reversibility
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.2132 S-adenosyl-L-methionine + guanine10 in tRNA depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures Pyrococcus abyssi 2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.2132 S-adenosyl-L-methionine + guanine10 in tRNA identity elements required for the efficient formation of N2-dimethylguanine10: uracil25 and a V-loop of four nucleotides are major determinants for the formation of N2-dimethylguanine10, whereas the identity of the 13-22 base-pair clearly influences the rate of N2-dimethylguanine10 formation. The integrity of the T-arm prolonged with the amino acid acceptor stem is an important structural element for the dimethylation step catalysed by PabTrm-m2 2G10 enzyme Pyrococcus abyssi 2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.213more model for a potential binding mode for the THUMP domain, which may be common to various RNA modification enzymes that specifically modify nucleotides in the 3D-core of the tRNA molecule Pyrococcus abyssi ? - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.213S-adenosyl-L-methionine + guanine10 in tRNA - Pyrococcus abyssi S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA - ?
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.213S-adenosyl-L-methionine + guanine10 in tRNA depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures Pyrococcus abyssi S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA - ?
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