EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.192 | 2 S-adenosyl-L-methionine + adenine in 155mer RNA |
- |
Clostridioides difficile |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine in 155mer RNA |
- |
? |
2.1.1.192 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
- |
Staphylococcus aureus |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA |
- |
? |
2.1.1.192 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
- |
Escherichia coli |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA |
- |
? |
2.1.1.192 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
- |
Escherichia coli K-12 |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA |
- |
? |
2.1.1.192 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
RlmN is an endogenous enzyme used by the cell to refine functions of the ribosome in protein synthesis |
Escherichia coli K-12 |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA |
- |
? |
2.1.1.192 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
the enzyme catalyzes the natural post-transcriptional modification of A2503 to m2A in Escherichia coli 23S rRNA |
Escherichia coli |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA |
- |
? |
2.1.1.192 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA |
S-adenosyl-L-methionine is both the methyl donor and the source of a 5'-deoxyadenosyl radical, which activates the substrate for methylation. The enzyme can utilize protein-free 23S rRNA as a substrate, but not the fully assembled large ribosomal subunit, suggesting that the methylations take place during the assembly of the ribosome. The key recognition elements in the 23S rRNA are helices 90-92 and the adjacent single stranded RNA that encompasses A2503 |
Escherichia coli K-12 |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA |
identification of the reaction product as 2-methyladenine2503 |
? |
2.1.1.192 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin |
- |
Staphylococcus aureus |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin |
- |
? |
2.1.1.192 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin |
- |
Escherichia coli |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin |
- |
? |
2.1.1.192 | 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin |
two S-adenosyl-L-methionine molecules serve as the cofactor by providing the 5'-deoxyadenosyl radical for substrate activation and the methyl |
Staphylococcus aureus |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin |
- |
? |