EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.183 | 4 S-adenosyl-L-methionine + adenine1779/1780 in 18S rRNA |
- |
Saccharomyces cerevisiae |
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA |
- |
? |
2.1.1.183 | 4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA |
- |
Saccharomyces cerevisiae |
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA |
- |
? |
2.1.1.183 | 4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA |
Dim1p is required for pre-ribosomal RNA processing |
Saccharomyces cerevisiae |
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA |
- |
? |
2.1.1.183 | 4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA |
presence of the 6-dimethyladenine1779/6-dimethyladenine1780 is detected by primer extension |
Saccharomyces cerevisiae |
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA |
- |
? |
2.1.1.183 | 4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA |
isolation of mutations that uncouple the enzymatic function of Dim1p in methylation from its involvement in prerRNA processing |
Saccharomyces cerevisiae |
4 S-adenosyl-L-homocysteine + N6-dimethyladenosine1779/N6-dimethyladenine1780 in 18S rRNA |
- |
? |
2.1.1.183 | 4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA |
neither the bacterial nor the archaeal ortholog could complement for the eukaryotic Dim1. This might be related to the secondary, non-methyltransferase function that Dim1 is known to play in eukaryotic ribosomal maturation |
Saccharomyces cerevisiae |
4 S-adenosyl-L-homocysteine + N6-dimethyladenosine1779/N6-dimethyladenosine1780 in 18S rRNA |
- |
? |
2.1.1.183 | more |
the enzyme catalyzed methylation of Escherichia coli 30S ribosomes. The enzyme produces a mixture of N6-methyladenine and 6-dimethyladenine (1.4 times as much N6-methyladenine as N6-dimethyladenine). 80% of the potential sites are methylated after 2 h. Under the assay conditions, ScDim1 releases the N6-methyladenine intermediate, which is subsequently converted to the N6-dimethyladenine product after an additional binding event |
Saccharomyces cerevisiae |
? |
- |
? |