EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.1.1.158 | more |
enzyme expression and activity during caffeine biosynthesis in fruits, overview |
Coffea canephora |
? |
- |
? |
2.1.1.158 | more |
enzyme expression and activity during caffeine biosynthesis in fruits, overview |
Coffea arabica |
? |
- |
? |
2.1.1.158 | more |
substrate specificity of the recombinant His-tagged enzyme, no activity with xanthosine 5'-phosphate, theobromine, or 7-methylxanthosine |
Coffea arabica |
? |
- |
? |
2.1.1.158 | more |
7-methylxanthosine synthase shows the most critical substrate specifi city of the three types of N-methyltransferases in Coffea species |
Coffea sp. |
? |
- |
? |
2.1.1.158 | more |
both xanthosine and its monoanionic form with N3 deprotonated are used as the substrates for the methylation. While the methyl group can be transferred to the monoanionic form of xanthosine with a reasonable free energy barrier (about 17 kcal/mol), this is not the case for the neutral xanthosine (barrier is about 31 kcal/mol). The hydrogen bonding and hydrophobic interactions involving the monoanionic form closely represent the corresponding interactions in the crystal structure, which seems not to be the case for the reactant complex involving the neutral xanthosine |
Coffea canephora |
? |
- |
- |
2.1.1.158 | more |
enzyme expression and activity during caffeine biosynthesis in fruits, overview |
Coffea canephora CCS1 |
? |
- |
? |
2.1.1.158 | S-adenosyl-L-methionine + xanthosine |
- |
Camellia sinensis |
S-adenosyl-L-homocysteine + 7-methylxanthosine |
- |
? |
2.1.1.158 | S-adenosyl-L-methionine + xanthosine |
- |
Coffea sp. |
S-adenosyl-L-homocysteine + 7-methylxanthosine |
- |
? |
2.1.1.158 | S-adenosyl-L-methionine + xanthosine |
- |
Coffea arabica |
S-adenosyl-L-homocysteine + 7-methylxanthosine |
- |
? |
2.1.1.158 | S-adenosyl-L-methionine + xanthosine |
- |
Coffea arabica |
S-adenosyl-L-homocysteine + 7-methylxanthosine |
- |
ir |