EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   1.14.99.48 | more |
an apparent meso-hydroxyheme is a likely common intermediate with the canonical heme oxygenases. Unlike heme oxygenases, this intermediate does not form with added H2O2 nor does it convert to verdoheme and CO. Rather, the next intermediates are a set of formyloxobilin isomers. These convert in separate fast and slow phases to beta-/delta-staphylobilin isomers and formaldehyde |
Staphylococcus aureus |
? |
- |
? |
| 1.14.99.48 | more |
dissociation equilibrium constants of heme are 1.4 nM and 12.9 nM for isoforms IsdG and IsdI, respectively. Heme dissociation rate constants are 0.022 per s for IsdG and 0.092 per s for IsdI. It is proposed that only IsdG is a member of the heme iron acquisition pathway and IsdI regulates heme homeostasis |
Staphylococcus aureus |
? |
- |
? |
| 1.14.99.48 | more |
the primary C1 product of the both isoform IsdG and IsdH is formaldehyde. In the mechanism, one meso carbon atom of the porphyrin ring is liberated primarily as formaldehyde |
Staphylococcus aureus |
? |
- |
? |
| 1.14.99.48 | more |
an apparent meso-hydroxyheme is a likely common intermediate with the canonical heme oxygenases. Unlike heme oxygenases, this intermediate does not form with added H2O2 nor does it convert to verdoheme and CO. Rather, the next intermediates are a set of formyloxobilin isomers. These convert in separate fast and slow phases to beta-/delta-staphylobilin isomers and formaldehyde |
Staphylococcus aureus N315 |
? |
- |
? |
| 1.14.99.48 | protoheme + 4 AH2 + 4 O2 |
- |
Staphylococcus aureus |
15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O |
enzyme cleaves the tetrapyrrol ring structure of heme in the presence of NADPH cytochrome P450 reductase, thereby releasing iron |
? |
| 1.14.99.48 | protoheme + 4 AH2 + 4 O2 |
- |
Staphylococcus aureus |
15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O |
oxidoreductase IruO, gene name NWMN2274, is the likely in vivo reductant required for heme degradation by Staphylococcus aureus. In the presence of NADPH and IruO, either IsdI or IsdG degrade bound heme 10fold more rapidly than with the chemical reductant ascorbic acid. The major heme degradation products are 5-oxo-delta-bilirubin and 15-oxo-beta-bilirubin, i.e. staphylobilins |
? |
| 1.14.99.48 | protoheme + 4 AH2 + 4 O2 |
- |
Staphylococcus aureus |
15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O |
oxidoreductase IruO, gene name thatNWMN2274, is the likely in vivo reductant required for heme degradation by Staphylococcus aureus. In the presence of NADPH and IruO, either IsdI or IsdG degrade bound heme 10fold more rapidly than with the chemical reductant ascorbic acid. The major heme degradation products are 5-oxo-delta-bilirubin and 15-oxo-beta-bilirubin, i.e. staphylobilins |
? |
| 1.14.99.48 | protoheme + 4 AH2 + 4 O2 |
- |
Staphylococcus aureus |
15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O |
product is a red-orange solid that becomes yellow when dissolved in dimethyl sulfoxide or water/acetonitrile. It is more polar than biliverdin and bilirubin |
? |
| 1.14.99.48 | protoheme + 4 AH2 + 4 O2 |
- |
Staphylococcus lugdunensis |
15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O |
heme degradation by IsdG results in the release of free iron and the production of the chromophore staphylobilin, i.e. 5-oxo-delta-bilirubin and 15-oxo-beta-bilirubin |
? |
| 1.14.99.48 | protoheme + 4 AH2 + 4 O2 |
- |
Staphylococcus aureus |
15-oxo-beta-bilirubin + Fe2+ + CO + 4 A + 4 H2O |
in the presence of NADPH and IruO, either IsdI or IsdG degrade bound heme 10fold more rapidly than with the chemical reductant ascorbic acid. The major heme degradation products are 5-oxo-delta-bilirubin and 15-oxo-beta-bilirubin, i.e. staphylobilins |
? |
