EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.20.15 | L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + 2 Cl- |
- |
Pseudomonas syringae pv. syringae |
4,4-dichloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O |
- |
? |
1.14.20.15 | L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + 2 Cl- |
- |
Pseudomonas syringae pv. syringae B301D |
4,4-dichloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O |
- |
? |
1.14.20.15 | L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Br- |
- |
Pseudomonas syringae pv. syringae |
4-bromo-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O |
- |
? |
1.14.20.15 | L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Br- |
- |
Pseudomonas syringae pv. syringae B301D |
4-bromo-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O |
- |
? |
1.14.20.15 | L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- |
- |
Pseudomonas syringae pv. syringae |
4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O |
- |
? |
1.14.20.15 | L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- |
the enzyme participates in syringomycin E biosynthesis |
Pseudomonas syringae pv. syringae |
4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O |
- |
? |
1.14.20.15 | L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- |
substrate positioning controls the partition between halogenation and hydroxylation |
Pseudomonas syringae pv. syringae |
4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O |
- |
? |
1.14.20.15 | L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- |
the enzyme can not chlorinate free L-Thr or the small molecule surrogate for L-Thr-S-SyrB1, L-Thr-S-N-acetylcysteamine |
Pseudomonas syringae pv. syringae |
4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O |
- |
? |
1.14.20.15 | L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- |
the substrate consists of L-Thr tethered via thioester linkage to a covalently bound phosphopantetheine cofactor of a carrier protein, SyrB1. Without an appended amino acid, SyrB1 does not trigger formation of the chloroferryl intermediate state in SyrB2, even in the presence of free L-Thr or its analogues, but SyrB1 charged either by L-Thr or by any of several non-native amino acids does trigger the reaction by as much as 8000fold (for L-Thr-S-SyrB1). Triggering efficacy is sensitive to the structures of both the amino acid and the carrier protein, being diminished by 5-20fold when the native L-Thr is replaced by another amino acid and by about 40fold when SyrB1 is replaced by a heterologous carrier protein, CytC2. The SyrB2 chloroferryl state exhibits unprecedented stability (t1/2 = 30-110 min at 0°C), can be trapped in high concentration and purity by manual freezing without a cryosolvent, and represents an ideal target for structural characterization |
Pseudomonas syringae pv. syringae |
4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O |
- |
? |
1.14.20.15 | L-threonyl-[L-threonyl-carrier protein SyrB1] + 2-oxoglutarate + O2 + Cl- |
the enzyme participates in syringomycin E biosynthesis |
Pseudomonas syringae pv. syringae B301D |
4-chloro-L-threonyl-[L-threonyl-carrier protein SyrB1] + succinate + CO2 + H2O |
- |
? |