EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.13.187 | dTDP-beta-L-evernosamine + 2 NADPH + 2 H+ + 2 O2 |
overall reaction |
Streptomyces peucetius |
dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + 2 NADP+ + 3 H2O |
- |
? |
1.14.13.187 | dTDP-beta-L-evernosamine + 2 NADPH + 2 H+ + 2 O2 |
overall reaction, dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose. The nitroso group is probably spontaneously oxidized to a nitro group giving dTDP-beta-L-evernitrose, which is involved in the biosynthesis of the antibiotic everninomycin |
Micromonospora carbonacea |
dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + 2 NADP+ + 3 H2O |
- |
? |
1.14.13.187 | dTDP-beta-L-evernosamine + 2 NADPH + 2 H+ + 2 O2 |
overall reaction, dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose. The nitroso group is probably spontaneously oxidized to a nitro group giving dTDP-beta-L-evernitrose, which is involved in the biosynthesis of the antibiotic everninomycin |
Micromonospora carbonacea var. africana |
dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + 2 NADP+ + 3 H2O |
- |
? |
1.14.13.187 | dTDP-beta-L-evernosamine + NADPH + H+ + O2 |
dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose |
Micromonospora carbonacea |
dTDP-N-hydroxy-beta-L-evernosamine + NADP+ + H2O |
- |
? |
1.14.13.187 | dTDP-beta-L-evernosamine + NADPH + H+ + O2 |
dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose |
Micromonospora carbonacea var. africana |
dTDP-N-hydroxy-beta-L-evernosamine + NADP+ + H2O |
- |
? |
1.14.13.187 | dTDP-N-hydroxy-beta-L-evernosamine + NADPH + H+ + O2 |
dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose |
Micromonospora carbonacea |
dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + NADP+ + 2 H2O |
- |
? |
1.14.13.187 | dTDP-N-hydroxy-beta-L-evernosamine + NADPH + H+ + O2 |
dTDP-beta-L-evernosamine i.e. dTDP-3-amino-2,3,6-trideoxy-3-C-methyl-4-O-methyl-beta-L-arabino-hexopyranose |
Micromonospora carbonacea var. africana |
dTDP-2,3,6-trideoxy-3-C-methyl-4-O-methyl-3-nitroso-beta-L-arabino-hexopyranose + NADP+ + 2 H2O |
- |
? |
1.14.13.187 | more |
thymine is recognized by interactions between the C4 carbonyl O atom and Arg332, and between both N3 and the C4 carbonyl O atom and a water molecule that hydrogen-bonds to the backbone carbonyl of Ala322. The ribose 3' hydroxyl group is hydrogen-bonded by Glu117, which is suitably positioned to form a salt bridge with Arg243. The dTDP diphosphate makes no apparent interactions with the protein |
Streptomyces peucetius |
? |
- |
? |
1.14.13.187 | TDP-L-epi-vancosamine + 2 NADPH + 2 H+ + 2 O2 |
- |
Streptomyces peucetius |
? + 2 NADP+ + 3 H2O |
the enzyme catalyzes oxidation of TDP-L-epi-vancosamine to its vicinal hydroxy nitroso congener triggers C-C bond cleavage. The overall rearrangement of the hydroxy nitroso sugar to the aldehyde-oxime product is formally that of a retro oxime-aldol transformation |
? |
1.14.13.187 | TDP-L-epi-vancosamine + 2 NADPH + 2 H+ + 2 O2 |
analog of TDP-L-evernitrosamine. Molecular oxygen, rather than oxygen from water, is incorporated into intermediates and products |
Micromonospora carbonacea |
? + 2 NADP+ + 3 H2O |
- |
? |